Green nanotechnology is expanding its frontier in the world of science and technology and has been termed "the miracle of science" by some researchers as it continues to provide solutions and alternatives to technological, environmental, energy and health challenges (^{Mata, Palmer, Tejeda-Montes, & Stupp, 2012}). Nanostructures are the matter of interest for all applications of Nanotechnology wherein shape and size of the nanoparticles (NPs) determine their characteristic properties. Due to the growing demand for various nanoparticles, it is necessary to develop synthetic routes that are cost-effective and environment-friendly. The majority of the existing procedures used for nanoparticle synthesis rely upon physical and chemical methods which could introduce toxic and hazardous materials into the system (^{Ai, Biazar, & Jafarpour, 2011; Mukherjee, Roy, & Mandal, 2008}) Moreover, the specific requirement for size and shape of nanoparticles cannot be met with the physico-chemical methods. Thus, biological methods involving microorganisms or plant extracts have shown to be more effective but in this recent research, there is a drift from the already known biological substrates to a new option which is plant sap. The integration of the principles of green chemistry to nanotechnology as regards to size manipulation of the nanoparticles is an unprecedented technique (^{Maryam, Niloofar, Ahmad, Fatemeh, & Sassan, 2012}).

• ^{Mata, Palmer, Tejeda-Montes, & Stupp, 2012}
  Design of biomolecules for nanoengineered biomaterials for regenerative medicine

  Methods in Molecular Biology, 2012
  Mata et al., 2012 Mata A, Palmer L, Tejeda-Montes E, Stupp S. Design of biomolecules for nanoengineered biomaterials for regenerative medicine. Methods in Molecular Biology. 2012; 811:39-49
• ^{Ai, Biazar, & Jafarpour, 2011; Mukherjee, Roy, & Mandal, 2008}
  Nanotoxicology and nanoparticle safety in biomedical designs

  International Journal of Nanomedicine, 2011
  Ai et al., 2011 Ai J, Biazar E, Jafarpour M. Nanotoxicology and nanoparticle safety in biomedical designs. International Journal of Nanomedicine. 2011; 6:1117-27
• ^{Maryam, Niloofar, Ahmad, Fatemeh, & Sassan, 2012}
  Extracellular production of silver nanoparticles by using three common species of dermatophytes: Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis

  Iranian Biomedical Journal, 2012
  Maryam et al., 2012 Maryam M, Niloofar R, Ahmad R.S, Fatemeh N, Sassan R. Extracellular production of silver nanoparticles by using three common species of dermatophytes: Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. Iranian Biomedical Journal. 2012; 16:52-8

Nanocomplexes of Schiff bases on the other hand are among the least areas of research in nanoscience that may hold great pharmaceutical applications. In our present world where new disease causing organisms such as Avian influenza, HIV/AIDS, Middle East respiratory syndrome (MERS), Ebola virus, etc., are discovered on daily basis, some being so tiny, posing difficulties in their disease treatment. It is reasonable to tackle these nano cum micro disease causing organisms with effective complex tools at nanoscale.

Kaushik in 2012, utilized grape juice to synthesis silver nanoparticles using different concentrations of the silver nitrate in the dark and at room temperature (^{Kaushik, 2013}). In his work, the SPB was noticed at 450 nm and the particle size of the nanoparticles was 18-20 nm. According to his reports, the nanoparticles exhibited high antimicrobial activities.

• ^{Kaushik, 2013}
  Green synthesis of silver nanoparticles by using grape fruit extract: Characterization of the particles & study of antibacterial activity

  Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2013
  Kaushik, 2013 Kaushik R. Green synthesis of silver nanoparticles by using grape fruit extract: Characterization of the particles & study of antibacterial activity. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2013; 4:1271-6

This present research focuses on using specific bio-molecules present in plant sap (sugarcane juice) as substrate to synthesize nanoparticles while anchoring it with Schiff base ligand to form a silver nanocomplex. The limitation of green silver nanoparticles and its antimicrobial activities vis-a-vis the strategies to improve the production of green antimicrobial drugs have also been discussed but not much information are available on nanocomplexes with Schiff base ligand. The development of nano-metal complexes by biological method is yet to be explored by researchers and on that note; it becomes a paramount objective in this study.

Moreover, in present times, clothing, respirators, household water filters, contraceptives, antibacterial sprays, cosmetics, detergent, dietary supplements, cell phones, laptop keyboards, and children's toys are among the products being marketed that purportedly exploit the antimicrobial properties of silver nanomaterials (^{Mohammad, Niloofar, & Marjan, 2013}). Apart from capitalizing on the antimicrobial potentials of the nano-form of silver, the focus upon the green methods of synthesis is of primary interest so as to counter the physiological and environmental peril associated with the use of chemicals for the fabrication and use of silver nanomaterials (^{Geoprincy, Vidhya Srii, Poonguzhali, Gandhi, & Renganathan, 2013}). Synthesis of green silver nanoparticles using plant extracts is a very simple and cost-effective way that satisfies the demand of the research community and simultaneously eliminates the possibility of environmental hazards; the plant sap seems to offer better opportunities though the results of this research will guide us appropriately. Our major aim therefore is to biologically synthesis silver nanoparticles using sugarcane juice while anchoring it with a Schiff base ligand.

• ^{Mohammad, Niloofar, & Marjan, 2013}
  Antibacterial properties of nanosilver finish cellulose fabric

  Journal of Nanostructure in Chemistry, 2013
  Mohammad et al., 2013 Mohammad M, Niloofar Y, Marjan M. Antibacterial properties of nanosilver finish cellulose fabric. Journal of Nanostructure in Chemistry. 2013; 3:43
• ^{Geoprincy, Vidhya Srii, Poonguzhali, Gandhi, & Renganathan, 2013}
  A review on green synthesis of silver nanoparticles

  Asian Journal of Pharmaceutical and Clinical Research, 2013
  Geoprincy et al., 2013 Geoprincy G, Vidhya Srii B.N, Poonguzhali U, Gandhi N.N, Renganathan S. A review on green synthesis of silver nanoparticles. Asian Journal of Pharmaceutical and Clinical Research. 2013; 6:8-12

The silver nanoparticles is synthesized using sugarcane sap while the ligand is a reaction between 3,4-dimethoxybenzaldehyde and 2-aminoethanesulfonic acid employing lemon juice as a green-catalytic approach.

Fresh sugarcanes (Sa. officinarum ) were collected from Okoripere village in Delta State, washed, peeled and chopped into smaller parts. It was further blended and filtered using Whatman filter paper and the filtrate used for the nanoparticle synthesis.

1000 ml of 1 mM AgNO₃ was mixed with 100 ml of surgarcane juice and stirred with a mechanical shaker at room temperature for 5 h.

The colorless sample was noticed to turn into light wine-red and the color intensified with time as shown in Figure 1. At different times; 0 h, 1 h, 2 h, and 5 h while stirring the solution at room temperature, they were sampled and analyzed using UV-vis spectrophotometer (^{Elemike et al., 2014; Sepideh, Seyedeh, Seyed, & Soheila, 2012}). It was observed that the surface plasmon band appeared at 475 nm after 1 h of reaction, showing the formation of silver nanoparticles and the spectra shown in Figure 2b.

• ^{Elemike et al., 2014; Sepideh, Seyedeh, Seyed, & Soheila, 2012}
  Evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf

  Micron, 2014
  Elemike et al., 2014 Elemike E.E, Oseghale C.O, Chuku A, Labulo A.H, Owoseni M.C, Mfon R, et al. Evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf. Micron. 2014; 57:1-5

In a 50 ml Erlenmeyer flask, 0.01 mole (1.710 g) 3,4-dimethoxybenzaldehyde and 0.01 mole (1.255 g) 2-aminoethanesulfonic acid was reacted in a 30 ml of deionised water/15 ml ethanol media mixture. Three drops of lemon juice was added as catalyst. The reacting mixture was stirred while heating at about 70 °C for 3 h (^{Ugwu & Okoro, 2014}). Light brown solution was observed, filtered and was left to stand for 2 days and crystals formed thereafter. The presence of the lemon juice catalyzed the reaction as without it, the solution could not crystallize for some days under observation (Scheme 1).

• ^{Ugwu & Okoro, 2014}
  Synthesis, characterization and antifungal activities of [4-methylphenylsulphonamido]-N-(pyridin-2-yl)acetamide derivatives

  Medicinal Chemistry, 2014
  Ugwu and Okoro, 2014 Ugwu D.I, Okoro U.C. Synthesis, characterization and antifungal activities of [4-methylphenylsulphonamido]-N-(pyridin-2-yl)acetamide derivatives. Medicinal Chemistry. 2014; 4:330-3

Synthesis of the silver nanocomplex was done by anchoring the ligand as prepared above on the synthesized silver nanoparticles (^{Sujarania, Sironmani, & Ramu, 2012}).

• ^{Sujarania, Sironmani, & Ramu, 2012}
  Synthesis, characterization and toxicity studies of Schiff bases [2-(2,2-diphenylethylimino)methyl)phenols] anchored silver nanoparticles

  Digest Journal of Nanomaterials and Biostructures, 2012
  Sujarania et al., 2012 Sujarania S, Sironmani T.A, Ramu A. Synthesis, characterization and toxicity studies of Schiff bases [2-(2,2-diphenylethylimino)methyl)phenols] anchored silver nanoparticles. Digest Journal of Nanomaterials and Biostructures. 2012; 7:1843-57

The ligand in a 30:15 ml water-ethanol ratio respectively was mixed with 10 ml of the aqueous silver nanoparticles solution prepared using sugarcane juice and little quantity of lemon juice added as catalyst. The entire solution was gently stirred for an hour at room temperature. Afterwards, it was left to stand for 2 days and the resultant light brown crystals were dried and characterized.

The absorption spectrum of the materials was measured on a T-60 UV-vis spectrophotometer and the spectra shown in Figure 2a-c. The phase identity and crystalline size of the nanoparticles and nanocomplex were characterized by X-ray diffractometer and the representative plot shown in Figure 4. Morphological features were studied by using Hitachi-7000 scanning electron microscope (SEM), and the functional groups and possible bioreductants determined by the 8500S Fourier transform infra-red spectrophotometer.

The synthesized ligand, nanoparticles and nanocomplex were screened against some clinical bacteria isolates obtained from Standard Diagnostics Laboratory Effurun using Agar well dilution method. Bacterial cultures were prepared and different concentrations of the test samples and the control drug (Ciprofloxacin) were equally prepared up to a minimum concentration of 20 μg/ml using standard methods (^{Nelson et al., 2007; Prabakaran, Subha, Thennarasu, & Merinal, 2012}). The growth of the microorganisms was monitored and the results are shown in Table 1 and Figure 5.

• ^{Nelson et al., 2007; Prabakaran, Subha, Thennarasu, & Merinal, 2012}
  Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment

  Journal of Biomedical Nanotechnology, 2007
  Nelson et al., 2007 Nelson D, Priscyla D, Marcato, Gabriel I.H.D, Oswaldo L.A, Elisa E. Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. Journal of Biomedical Nanotechnology. 2007; 3:203-8

The substrate or biomolecules used for synthesis of nanoparticles play very important role in the surface reactions, size and morphology of nanoparticles. As is well known, biomolecules can carry on slow kinetics to self-reduce metal precursors and develop stable coating layers to avoid particle aggregation (^{Renquan, Dapeng, Daxiang, Zhongyang, & Lin, 2012}).

• ^{Renquan, Dapeng, Daxiang, Zhongyang, & Lin, 2012}
  Egg white mediated green synthesis of silver nanoparticles with excellent biocompatibility and enhanced radiation effects on cancer cells

  International Journal of Nanomedicine, 2012
  Renquan et al., 2012 Renquan L, Dapeng Y, Daxiang C, Zhongyang W, Lin G. Egg white mediated green synthesis of silver nanoparticles with excellent biocompatibility and enhanced radiation effects on cancer cells. International Journal of Nanomedicine. 2012; 7:2101-7

From Figure 1, it is seen that the color started changing gradually to wine-red and this was confirmed by surface plasmon bands displayed by the nanoparticles within 1 h of the reaction at a wavelength of about 475 nm. This wavelength persisted even at the subsequent periods of sampling showing that the substrate (sugarcane juice) acts as both reducing and stabilizing agent as shown in Figure 2b.

The major component of sugarcane juice that has caused the bio-reduction of Ag⁺ to Ag⁰ is glucose and other components such as proteins, minerals etc. which are in minor quantities. Sucrose is the major component of cane juice but may not have affected the reduction since it is not a reducing agent. In fact, the same molecular mechanisms that give antioxidant properties to these molecules must have promoted the reduction of Ag⁺ ions to Ag atoms. The exact mechanism of the extracellular biosynthesis of metal nanoparticle is not well understood. However, two possible mechanisms have been hypothesized. These are; hydrogen abstraction, due to the OH groups in the sucrose molecules and redox reaction of nicotinamide adenine dinucleotide (NADH) coenzyme which works as an electron shuttle to neutralize Ag⁺ ion (^{Labrenz et al., 2000; Roh et al., 2001; Swarnalatha, Christina, Shruti, & Payas, 2012}). For the formation of silver nanoparticles, the sample changed its visual appearance within 1 h after addition of the extract, indicating that a reduction reaction took place. Initially, the reacting mixture was slightly brownish liquid; as the reaction proceeded, the solution became deep wine-red showing the strong absorption of visible light due to excitation of the nanoparticle surface plasmons (^{Amendola, Bakr, & Stellacd, 2010; Ericka et al., 2012; Hartland, 2011; Kwon, Lee, Wark, & Lee, 2012; Ray, 2010}). Figure 2b shows that the SPBs was formed within 1 h and stabilized even after the 5th hour of sampling. The sugarcane extract did not give bands within the range of 400-500 nm which is a clear evidence that it is not nanoparticles. The surface plasmon absorption in the nanoparticles is due to the collective oscillation of the free conduction band electrons which is excited by the incident electromagnetic radiation. This type of resonance is seen when the wavelength of the incident light far exceeds the particle diameter (^{Naika et al., 2015}).

• ^{Labrenz et al., 2000; Roh et al., 2001; Swarnalatha, Christina, Shruti, & Payas, 2012}
  Formation of sphalerite (ZnS) deposits in natural biofilms of sulfate-reducing bacteria

  Science Journal, 2000
  Labrenz et al., 2000 Labrenz M, Druschel G.K, Thomsen E.T, Gilbert B, Welch S.A, Kemner K.M. Formation of sphalerite (ZnS) deposits in natural biofilms of sulfate-reducing bacteria. Science Journal. 2000; 1:744-7
• ^{Amendola, Bakr, & Stellacd, 2010; Ericka et al., 2012; Hartland, 2011; Kwon, Lee, Wark, & Lee, 2012; Ray, 2010}
  A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: Effect of shape, size, structure, and assemble

  Plasmonics, 2010
  Amendola et al., 2010 Amendola V, Bakr O.M, Stellacd F. A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: Effect of shape, size, structure, and assemble. Plasmonics. 2010; 5:85-97
• ^{Naika et al., 2015}
  Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity

  Journal of Talibah University of Science, 2015
  Naika et al., 2015 Naika H.R, Lingaraju K, Manjunath K, Danith K, Nagaraju G, Suresh D, et al. Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity. Journal of Talibah University of Science. 2015; 9:7-12

The nature of the broad band shows that the nanoparticles are polydisperse and have greater distribution of particle size (^{Hale, Maddox, Shapter, & Voelcker, 2005}). Moreover, the constant nature of the bands in the subsequent reaction times proves the stable nature of the nanoparticles formed thereby reassuring the substrate as a good bio-reducing and stabilizing agent.

• ^{Hale, Maddox, Shapter, & Voelcker, 2005}
  Growth kinetics and modeling of ZnO nanoparticles

  Journal of Chemical Education, 2005
  Hale et al., 2005 Hale P.S, Maddox L.M, Shapter J.G, Voelcker N.H. Growth kinetics and modeling of ZnO nanoparticles. Journal of Chemical Education. 2005; 82:775-8

In the case of the Schiff base ligand, absorption bands were observed at 310 nm, 280 nm and 230 nm which could be attributed to the chromophores in the ligand.

In Figure 2c, the spectra of the nanocomplex showed a broad peak with a maximum absorption at 450 nm. The blue shift in the surface plasmon bands as compared to Figure 2b is an indication of smaller colloids equally supported by the broader peak which also indicates a greater distribution of the particle size.

Figure 3a-c shows the FTIR spectra of 2-imino-(3,4-dimethoxybenzyl) ethanesulfonic acid Schiff base, silver nanoparticles and nanocomplex. The absence of spikes above 3300 cm⁻¹ in Figure 3a show that the primary amine has formed an imine in the ligand and cannot be absorbed within that frequency range (^{Wade, 2006}). The weak absorption around 3442 cm⁻¹ could be attributed to some water molecules. The strong absorption peaks at 2939 and 2841 cm⁻¹ for the ligand and 2357 cm⁻¹ for the nanocomplex indicates the CH stretching vibrations. The imine (CN) functional group absorbed strongly at 1679 cm⁻¹ for the ligand and at 1678 cm⁻¹ for the nanocomplex. The presence of the benzene ring conjugated to imine group in both the ligand and nanocomplex were noticed at 1589 and 1590 cm⁻¹ respectively (^{Okoli, Adewuyi, Zhang, Diagboya, & Guo, 2014}). The striking feature of SO₃H group was shown by the absorptions at 1259 cm⁻¹ and 1256 cm⁻¹ for the ligand and nanocomplex respectively. The CO stretching vibrations which is characteristic of ether groups were observed at 1018, 1135 cm⁻¹ for the ligand and 1025, 1133 cm⁻¹ for the nanocomplex. The strong absorption at 360 cm⁻¹ in the nanocomplex which is absent in the Schiff base ligand is a strong indication of presence of silver metal. Figure 3b which shows the spectra of the silver nanoparticles has weak absorptions at 3386 cm⁻¹ which indicates OH molecules. The other absorptions at 1650 cm⁻¹ and 1041 cm⁻¹ are attributed to CO bonds probably due to glucose molecules while the band at 386 cm⁻¹ represents the silver metal (^{Morrison & Boyd, 2002}).

• ^{Wade, 2006}
  Organic chemistry, 2006
  Wade, 2006 Wade L.G. Organic chemistry. 6th ed.. USA: Prentice and Hall; 2006. 523-32
• ^{Okoli, Adewuyi, Zhang, Diagboya, & Guo, 2014}
  Mechanism of diakyl phthalates removal from aqeous solution using γ-cyclodextrin and starchbased polyurethane polymer adsorbents

  Carbohydrates Polymers, 2014
  Okoli et al., 2014 Okoli P.C, Adewuyi G.O, Zhang Q, Diagboya P.N, Guo Q. Mechanism of diakyl phthalates removal from aqeous solution using γ-cyclodextrin and starchbased polyurethane polymer adsorbents. Carbohydrates Polymers. 2014; 114:440-9
• ^{Morrison & Boyd, 2002}
  Organic chemistry, 2002
  Morrison and Boyd, 2002 Morrison R.T, Boyd R.N. Organic chemistry. 6th ed.. Prentice and Hall India; 2002. 590-2

The XRD diffraction pattern of dry nanosilver powder in Figure 4 shows a crystalline nanocomplex of about 15 nm with the peaks suggesting high purity of the compound. The other observed peak and noise were probably related to the effect of nanosized particles and the presence of various crystalline biological macromolecules in the sugarcane sap. The obtained results illustrate that silver ions had indeed been reduced to Ag by the sap and ligand under reaction conditions (^{Forough & Farhadi, 2010; Rajesh, Raja, Rathi, & Sahayaraj, 2012}). The nanoparticles showed higher particle sizes of about 25-30 nm.

• ^{Forough & Farhadi, 2010; Rajesh, Raja, Rathi, & Sahayaraj, 2012}
  Biological and green synthesis of silver nanoparticles

  Turkish Journal of Engineering and Environmental Sciences, 2010
  Forough and Farhadi, 2010 Forough M, Farhadi K. Biological and green synthesis of silver nanoparticles. Turkish Journal of Engineering and Environmental Sciences. 2010; 34:281-7

The SEM showed interesting morphologies both for the sugarcane nanoparticles and the nanocomplex as shown in Figure 5a-d. Figure 5a showed an intermediate morphology between cube and cone that may have potential applications as molecular grippers in nanomedicine.

Based on the micrographs and supporting UV-vis spectra, the early stages of growth of the nanoparticles could be seen to follow the oriented attachment (OA) since the nanocrystallites grow on the already existing nanocubes followed by surface crystallization and a phase transformation to an intermediate cube-cone nanostructures as shown in Figure 5a.

The OA mechanism has attracted preponderant interests due to its fundamental role in designing and exploring materials with size and morphology controllability (^{Zhang, Huang, & Lin, 2010}). Peculiar structures and morphologies displayed by the nanosystems are due to reduction in surface energy and coherent crystallographic orientation which provides a route for the incorporation of defects like stacking faults, twins and misorientation.

• ^{Zhang, Huang, & Lin, 2010}
  Progress of nanocrystalline growth kinetics based on oriented attachment

  Nanoscale, 2010
  Zhang et al., 2010 Zhang J, Huang F, Lin Z. Progress of nanocrystalline growth kinetics based on oriented attachment. Nanoscale. 2010; 2:18-34

The anchoring of ligand on the nanocubes and cones seems to affect the crystal growth kinetics of the system thereby giving rise to the small spherical nanoparticles exhibited by the nanocomplex as shown in Figure 5c and d. The introduction of the organic ligand which has unstable surface adsorption property could be destroyed or desorbed in the hydrothermal medium. Evidently, the appearance of the small spherical particles in the nanocomplex indicates that the mass transportation was incessant. There was further crystallization which extended inwards at the expense of the ligand nutrients creating large interior space and surface area (^{Greer, 2014}). This could equally be supported by the UV-vis spectra shown in Figure 2a and c where there was a blue shift from 475 nm in the nanoparticles to 450 nm in the nanocomplex. It would be expected that the Ostwald ripening should have set in since the ligand molecules may not be able to penetrate into the core region of the nanosystems but based on the evidence from the results, digestive ripening which is the inverse of Ostwald ripening could be suggested as the growth mechanisms (^{Greer, 2014}). The process of formation is controlled by the surface energy of the particle within solution where the larger particles redissolve and the smaller ones grow (^{Nguyen, Maclean, & Mahiddine, 2014}). Moreso, the diffusion of the ligands along the surface of the nanosystems could have changed the shape of the particle. This situation occurs under specific conditions where the energy of the ligands within the solution is probably low, forming a gap between the shell and the core particle (^{Yanjun et al., 2012}).

• ^{Greer, 2014}
  Non classical crystal growth of inorganic and organic materials

  Material Science and Technology, 2014
  Greer, 2014 Greer H.F. Non classical crystal growth of inorganic and organic materials. Material Science and Technology. 2014; 30:611-25
• ^{Greer, 2014}
  Non classical crystal growth of inorganic and organic materials

  Material Science and Technology, 2014
  Greer, 2014 Greer H.F. Non classical crystal growth of inorganic and organic materials. Material Science and Technology. 2014; 30:611-25
• ^{Nguyen, Maclean, & Mahiddine, 2014}
  Mechanisms of nucleation and growth of nanoparticles in solution

  Chemical Reviews, 2014
  Nguyen et al., 2014 Nguyen T.K.T, Maclean N, Mahiddine S. Mechanisms of nucleation and growth of nanoparticles in solution. Chemical Reviews. 2014; 114:7610-30
• ^{Yanjun et al., 2012}
  Formation mechanism of hollow microspheres consisting of ZnO nanosheets

  CrystEngComm, 2012
  Yanjun et al., 2012 Yanjun F, Zhenbo X, Fengjiao Y, Jian S, Yewu W, Wuzong Z. Formation mechanism of hollow microspheres consisting of ZnO nanosheets. CrystEngComm. 2012; 14:8615-9

The type of kinetics displayed by the sugarcane nanoparticles based on the spectral and micrograph evidence is a typical oriented attachment. Moreover, this experimental result is in consonance with the theoretical as well as other experimental studies which suggest that growth of nanoparticles can be greatly influenced by surface reactions thereby proceeding from a diffusion controlled mechanism onwards to oriented attachment (^{Dare et al., 2015}). It could be suggested that the surface adsorption of the capping organic molecules (such as sucrose) present in the sugarcane sap, which was not involved in bioreduction could have been purely responsible for the exclusive OA growth as shown in Scheme 2. According to Penn, OA mechanism is due to the degree of electrostatic interactions such as the Vander waal forces which consequently determines the kinetic rate constant (^{Penn & Banfield, 2004}). While ^{Ribeiro, Lee, Longo, and Leite, 2005}, considers the diffusion and coagulation of the colloids thereby suggesting that the viscosity of the medium played a significant role in the OA mechanism, which is governed by an inverse proportional relationship with respect to the rate constant. In the above-mentioned models, only primary particles are assumed to combine to give secondary particles and the reaction stops; but experimentally, the OA mechanism has proven that growth behavior can go beyond collision and reaction of molecules. The nanocrystals may be capped with easily destroyed and desorbed organic ligands with the coalescence of some multilevel particle being neglected. This mechanism can be likened to the classical Smoluchowski equation which is a limiting case of aggregation that involves monomer-monomer and monomer-multimer reactions neglecting the multimer-multimer reactions.

• ^{Dare et al., 2015}
  Green synthesis and growth kinetics of nanosilver under bio-diversified plant extracts influence

  Journal of Nanostructure in Chemistry, 2015
  Dare et al., 2015 Dare E.O, Oseghale C.O, Labulo A.H, Adesuji E.T, Elemike E.E, Onwuka J.C, et al. Green synthesis and growth kinetics of nanosilver under bio-diversified plant extracts influence. Journal of Nanostructure in Chemistry. 2015; 5:85-94
• ^{Penn & Banfield, 2004}
  Kinetics of orientated aggregation

  Journal of Physical Chemistry B, 2004
  Penn and Banfield, 2004 Penn R.L, Banfield J.F. Kinetics of orientated aggregation. Journal of Physical Chemistry B. 2004; 108:12707-12
• ^{Ribeiro, Lee, Longo, and Leite, 2005}
  A kinetic model to describe the nanocrystal growth by the oriented attachment mechanism

  ChemPhysChem, 2005
  Ribeiro et al., 2005 Ribeiro C, Lee E.J.H, Longo E, Leite E.R. A kinetic model to describe the nanocrystal growth by the oriented attachment mechanism. ChemPhysChem. 2005; 6:690-5

The incorporation of organic ligands into the nanoparticle medium seems to arrest the OA growth process and introduces the digestive ripening. The polydisperse nanocrystal lattices emanating from the initial sugarcane mediated system was modified upon refluxing with ligand molecules leading to digestive ripening and nucleating the colloid at the phase boundary (^{Yijun, Deepa, Christopher, & Kenneth, 2013}). Unlike the well-known Ostwald ripening in which large particles grow at the expense of small particles, this process narrows the distribution by sharing material among different sizes of particles until a thermodynamically stable size segregation can further select the specific particle size (^{Lin, Sorensen, & Klabunde, 2000}). Many works have been reported on the stabilization of nanoparticles suggesting that control may be due to thermodynamics other than the kinetics of nucleation and crystal growth (^{Chikan & Kelley, 2002}). In this work, the nanoparticle size resulted from a combination of the ligand-silver binding energy and the surface free energy of the particles to reach the minimum energy of the whole system. The ligand-silver binding energy favors smaller particles with larger surface areas, while the surface free energy favors larger particles with smaller surface curvature. Therefore, these two effects oppose each other and led to a minimum energy with a thermodynamically favored size for each silver-ligand colloid system.

• ^{Yijun, Deepa, Christopher, & Kenneth, 2013}
  Alkyl and aromatic amines as digestive ripening/size focusing agents for gold nanoparticles

  Nanomaterials, 2013
  Yijun et al., 2013 Yijun S, Deepa J, Christopher S, Kenneth J.K. Alkyl and aromatic amines as digestive ripening/size focusing agents for gold nanoparticles. Nanomaterials. 2013; 3:370-92
• ^{Lin, Sorensen, & Klabunde, 2000}
  Digestive ripening, nanophase segregation and superlattice formation in gold nanocrystal colloids

  Journal of Nanoparticle Research, 2000
  Lin et al., 2000 Lin X.M, Sorensen C.M, Klabunde K.J. Digestive ripening, nanophase segregation and superlattice formation in gold nanocrystal colloids. Journal of Nanoparticle Research. 2000; 2:157-64
• ^{Chikan & Kelley, 2002}
  Size dependent spectroscopy of MoS2 nanoclusters

  Journal of Physical Chemistry B, 2002
  Chikan and Kelley, 2002 Chikan V, Kelley D.F. Size dependent spectroscopy of MoS2 nanoclusters. Journal of Physical Chemistry B. 2002; 106:3794-804

From the results, we could draw a conclusion that iminosulphonic acid does play a significant role as a capping ligand in stabilizing the nanocomplex and giving smaller particle sizes.

The ligand, silver nanoparticles and the nanocomplex were screened against Escherichia coli , Staphylococcus auerus and S. aubeus bacterial strains and the results are presented in Table 1. Using Agar well dilution method, the ligand, Ag Nps and nanocomplex showed significant antibacterial activity on all the three bacterial strains. Nano-silver inside bacterial cells disrupts the biochemical processes by binding with DNA molecules which leads to disordering of the helical structure by cross-linking within and between the nucleic acid strands (^{Kim, Cho, Ryu, & Choi, 2000}). Indirect effects through changes in the surrounding charge environment also have an impact on the effectiveness of nanoparticulate metals against microorganisms (^{Stohs & Bagchi, 1995}). Moreover, the silver released from the nanoparticles get attached to the negatively charged bacterial cell wall, rupturing it thereby leading to protein denaturation and finally causes cell death (^{Sondi & Sondi, 2004}). It is evident from Table 1 and Figure 6, that the growth of the three bacterial strains was severely inhibited by the nanocomplex than the ligands and nanoparticles.

• ^{Kim, Cho, Ryu, & Choi, 2000}
  Effects of metal ions on the activity of protein tyrosine phosphatase VHR: Highly potent and reversible oxidative inactivation by Cu2+ ion

  Archives of Biochemistry and Biophysics, 2000
  Kim et al., 2000 Kim J.H, Cho H, Ryu S, Choi M. Effects of metal ions on the activity of protein tyrosine phosphatase VHR: Highly potent and reversible oxidative inactivation by Cu2+ ion. Archives of Biochemistry and Biophysics. 2000; 382:72-80
• ^{Stohs & Bagchi, 1995}
  Oxidative mechanisms in the toxicity of metal ions

  Free Radical Biology and Medicine, 1995
  Stohs and Bagchi, 1995 Stohs J, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radical Biology and Medicine. 1995; 18:321-36
• ^{Sondi & Sondi, 2004}
  Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria

  Journal of Colloid and Interface Science, 2004
  Sondi and Sondi, 2004 Sondi I, Sondi B.S. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science. 2004; 275:177-82

From the research work, we have synthesized a Schiff base derived from 3,4-dimethoxybenzaldehyde and 2-aminoethanesulfonic acid and its silver nanocomplex. The formation of the nanocomplex, the ligand and the silver nanoparticles were analyzed using UV-vis spectrophotometer, FTIR, XRD and SEM. The ligand introduced digestive ripening on the nanoparticles framework thereby establishing stable spherical structures with high surface area and mechanical strength. The ligand with typical imine sulphonic acid group act as useful capping and stabilizing agent for silver nanoparticles while the refluxing close to the boiling point of water effected good digestive ripening. The antibacterial activities results shows that the nanocomplex has enhanced antibacterial properties on E. coli, S. aureus and S. aubeus than its parents Schiff base and nanoparticles. The greater efficacy of the nanocomplex is positively modified upon coordination of the ligand with the nano-silver metal. The established structure in this research therefore acts as self sacrificial templates to fabricate other structural nanosystems with enhanced functionalities. There is scarce information on the synthesis of silver nanocomplex. The results of this study have furnished us with some information but we need more analysis and further research to establish the unfolding success believed to be associated with this emerging bionanoparticulate inorganic system.

The authors have no conflicts of interest to declare. All authors participated in this research and have endorsed the publication of this work.