Introduction

“Herbal medicines are in widespread use and although many believe that herbal medicines are safe, and often used in combination are drawn from plant sources with their own variability in species, growing conditions, and biologically active constituents” [¹]. “The essential oils are natural products that plants produce for their own needs other than nutrition (i.e. protection or attraction). In general, they are complex mixtures of organic compounds that give characteristic odor and flavor to the plants. They are mainly made up of monoterpenes and sesquiterpenes whose main metabolic pathway is through mevalonate leading to sesquiterpenes and from methyl-erythritol leading to monoterpenes” [²]. The exploration of traditional knowledge for the cure to common diseases is attractive since antiquity. The medicinal plants are responsible for most of the medicine and food used in modern society [³]. In India, about70 percent of the rural population depends on the traditional Ayurvedic system of medicine. Most healers/practitioners of the traditional systems of medicine prepare formulations by their own recipes and dispense to the patients. The Western Ghats region is one of the 34 global biodiversity hotspots [⁴] which covers the Northwest Karnataka region.

Blumea lanceolaria (Roxb.) Druce. is belongs to the family Asteraceae. The leaves of B. lanceolaria are aromatic and used as a flavoring agent for the food. In traditional medicine, the leaves are used for the treatment of bronchitis, aphthae and asthma [⁵]. B. lanceolaria locally known as ‘Buarze’ in Mizoram, India and in folklore medicine this plant is used as an anti-cancer agent and leaves juice is useful for wound healing, chronic ulcers and infusion of leaves to control dysentery [⁶]. In the previous report, essential oil of B. lanceolaria has been reported a high amount of p-cymene [⁷] and methyl thymol [⁸] from India and Vietnam, respectively.

Heliotropium indicum L. of the family Boraginaceae is a high valued medicinal plant known in Ayurveda as Hastishundi Shrihastini or Vrischikaali and distributed throughout India [⁹]. This is one of the most widely used herbs in the Ayurvedic medicine of India and some other countries for the treatment of liver diseases [¹⁰]. In traditional medicine, this plant is used as a local application for ulcers, wounds, sores, gum boils and skin affections [⁹]. The tribal community of Phulbani district of Odisha, India use the leaf paste of H. indicumover fresh cuts and wounds and claim for its promising activity [¹¹] and scientifically reported wound healing activity on animals [¹¹,¹²]. The decoction of leaves is used in urticaria and fevers and root is used for the treatment of coughs. Small doses of flower decoction are used for emmenagogue, while abortifacient in large doses [⁹]. The pharmacological activities like gastroprotective, antimicrobial, [¹³], anti-inflammatory [¹⁴], activities have been reported from various solvent extracts of H. indicum. The alkaloids viz., indicine [¹⁰], indicinine [¹⁵], helindicine, lycopsamine [¹⁶], pestalamide B, glycinamide,N-(1-oxooctadecyl) glycyl-L-alanylglycyl-L-histidyl [¹⁷] and indicine-N-oxide [¹⁸] have been isolated from H. indicum. The major volatile compounds phytol, 1-dodecanol, and β-linalool have been reported from Thailand and the oil showed significant anti-tuberculosis activity against Mycobacterium tuberculosis H37Ra in the Alamar blue assay system with a MIC of 20.8 μg/mL [¹⁹]. Another report from Nigeria reported phenylacetaldehyde, (E)-2-nonenal and (E,Z)-2-nonadienal and hexahydrofarnesylacetone [²⁰] were the major constituents.

Triumfetta rhomboidea Jacq. of the family Tiliaceae is commonly known in English as ‘Burbush’ or ‘Burweed’; in Ayurveda as ‘Jhinjhireetaa’ or ‘Gippit’ and in Karnataka ‘Bankitutturi’ and distributed throughout tropical and subtropical India, up to 1200 m in the Himalayas [⁹]. The plant is known as a weed, but a number of medicinal properties have been described for its different parts. In eastern Africa, T. rhomboidea is used in folk medicine for its abortive properties on the gravid mammalian uterus [²¹] and treatment of cancer among the tribal population in Kolli Hills, South India [²²]. In traditional medicine, the bark and leaves of this plant are used for the treatment of diarrhea and dysentery [⁹]. Some pharmacopeia in eastern Africa described the uses of powdered leaves as an infusion for the treatment of anemia. In Kenya, this species is crushed and employed in massage to relieve labor pain. The infusion solution from its pounded roots is utilized as an anti-venom by applying to the bite area. In Rwanda, T. rhomboidea is used to treat pneumonia, otitis, and diarrhea [²³]. Various extracts of T. rhomboidea have been reported to possess cytotoxic [²³], antioxidant [²⁴] and antimicrobial [²¹] activities. A new compound triumfettamide B, along with lupeol, β-sitosterol, stigmasterol, oleanolic acid, maslinic acid, β-sitosterol glucopyranoside, and trans-tiliroside have been isolated and identified from T. rhomboidea [²³]. The aerial parts of T. rhomboidea have been reported trans-β-caryophyllene, kessane and caryophyllene oxide as the major constituents [²¹].

This communication presents the essential oil composition of the different species of the traditional herbs viz., B. lanceolaria, H. indicum and T. rhomboidea (Fig. 1) growing Western Ghats region of India with the aim of possible utilization of the oil and demarcation of its terpenoid profile.

Fig. 1 Plants species of the traditional herbs growing in Western Ghats of North West Karnataka. 

Experimental

Results and discussion

Chemical components of essential oils from B. lanceolaria, H. indicum, and T. rhomboidea have been presented in Table 1 in elution order from the BP-1 column, along with the percentage composition of each component and its retention index. Thirty-six, twenty-seven, and fifty constituents from the essential oils of B. lanceolaria, H. indicum, and T. rhomboidea, respectively were identified according to their mass spectra and their relative retention indices determined in a nonpolar stationary phase capillary column. The essential oils of B. lanceolaria, H. indicum, and T. rhomboidea comprising 97.3%, 98.2%, and 97.6%, respectively of the total oil constituents. The major compound of the essential oil of B. lanceolaria was identified as β-pinene (82.3%) along with other minor constituents terpin-4-ol (4.1%), γ-terpinene (2.5%) and sabinene (2.2%). The oil was found to be rich in monoterpene hydrocarbons (90.4%), followed by oxygenated monoterpenes (6.5%) and phenyl derivatives (0.2%). The major constituent of the essential oil of H. indicum was methyl salicylate (54.3%). The other minor constituents were sabinene (7.9%), eugenol (6.3%) and cis-calamenene (4.7%). The oil was found to be rich in phenylpropanoid type constituents (72.8%), followed by monoterpene hydrocarbons (17.6%), sesquiterpene hydrocarbon (6.6%), oxygenated monoterpene (0.9%) and oxygenated sesquiterpene (0.3%). The major compound of the essential oil of T. rhomboidea was identified as β-caryophyllene (28.9%). The other minor constituents were α-humulene (8.5%), germacrene D (6.9%), cis-calamene-10-ol (6.5%), α-cadinol (5.4%) and δ-cadinene (4.8%). The oil was found to be rich in sesquiterpene hydrocarbons (69.5%), followed by oxygenated sesquiterpenes (21.5%), oxygenated long-chain hydrocarbons (3.7%), monoterpene hydrocarbon (1.6%), and phenyl derivatives (1.3%) type constituents. The compounds terpin-4-ol (0.1-4.1%) and eugenol (t-6.3%) have been commonly identified in the essential oils of B. lanceolaria, H. indicum, and T. rhomboidea.

The essential oil constituents of B. lanceolaria growing in the Western Ghats region of India has somehow resembled the plant grown in Vietnam and other regions of India. The chemical constituents of the plant B. lanceolaria from Vietnam viz., α-thujene (0.04%), α-pinene (0.01%), camphene (0.01%), β-pinene (0.01%), α-terpinene (0.01%), p-cymene (3.28%), limonene (0.12%), methyl thymol (94.96%), and β-caryophyllene (0.04%) have been common to this report in quantitative divergence and methyl carvacrol (0.02%) and 1-hexadecanol (0.79%) which were reported from Vietnam [⁸] are not detected in this report. The rest of the compounds identified in this report are not identified in Vietnam grown B. lanceolaria (Table 1). Similarly, the only compound p-cymene (99%) has been reported from India in the very high amount [⁷] and this report this compound has been identified in trace (<0.1%).

A comparison of essential oil constituents of H. indicum of this study with the compounds of plant growing in Thailand [¹⁹] was found to be completely different. Apart from this, the report from Nigeria grown H. indicum plant only three compounds viz., methyl salicylate (1.2%), (E)-β-ionone (1.9%) and eugenol (2.4%) [²⁰] were found to be common compared to the present study.

The essential oil constituents reported [²¹] from the aerial parts of T. rhomboidea in terms of the major compound was similar to that found in this study with quantitative differences, while qualitatively relative amounts of the components which are somehow dissimilar from France growing plant. Correlation of the compounds from France growing plant with this study revealed that a high amount of β-caryophyllene and low amount of caryophyllene oxide were found in raw seeds oil of T. rhomboidea. It is interesting to note that the compound kessane which was the second major compound from France growing T. rhomboidea could not be detected even trace amount in the raw seed oil in this study. Overall, out of fifty compounds identified in the raw seeds of T. rhomboidea, twenty-one compounds were found to be similar with varying quantities as compared to the aerial parts of France growing T. rhomboidea plant [²¹]. Interestingly, the cumulative quantity of twenty-one similar constituents of raw seeds and aerial parts contains more or less similar quantities (65.4% from T. rhomboidea raw seeds oil and 65.7% from aerial parts T. rhomboidea oil.

The compound β-pinene possesses various biological activities viz., anti-infectious bronchitis virus [³²], antinociceptive [³³], antimicrobial [³⁴] as well as antiviral against herpes simplex virus [³⁵] and β-pinene present in high amount in the leaves of B. lanceolaria may support the traditional uses of this herb for the treatment of bronchitis, aphthae, asthma [⁵], wound healing, chronic ulcers, and to control dysentery [⁶].

The compound methyl salicylate in a small amount has been used as a topical counterirritant for the treatment of mild aches and pains of arthritis, as well as for acute muscle strain associated with the “weekend warrior” syndrome [³⁶]. Hence, the plant H. indicum used as local application support traditional medicine.

The β-caryophyllene is the main compound of the essential oil of T. rhomboidea and β-caryophyllene has anti-inflammatory [³⁷], anticancer [³⁸], antinociceptive [³⁹], cytoprotective [⁴⁰], analgesic [³⁸,⁴¹] and showed marked protective [⁴²] effects. The β-caryophyllene is an excellent therapeutic agent and the use of T. rhomboidea in traditional medicine may attribute to the treatment of various ailments like the treatment of cancer, diarrhea, gonorrhea to stop the burning caused by urine, treatment of anemia, relieve labor pain, etc.

The quantitative and qualitative divergence may be due to the geographical, climatic, and soil conditions, which in turn may affect the composition and other secondary metabolites of the plants [⁴³,⁴⁴]. Essential oil formation in the plants is highly dependent on climatic conditions, especially day length, irradiance, temperature, and water supply. Tropical species follow in their vegetation cycle the dry and rainy seasons, while species of the temperate zones react more on day length, the more distant from the equator their natural distribution area is located [⁴⁵]. The ecological experiments regard to the essential oil content was found a correlation between flower formation, flowering period, and essential oil synthesis [⁴⁵]. It was observed that the shorter the flowering phase, the less was the time available for oil formation, and thus the lower oil content. The composition of the essential oil, on the other hand, showed no qualitative change due to ecological or climatic factors confirming that chemotypes keep their typical pattern [⁴⁵].

Conclusion

The present investigation concludes that the essential of H. indicum collected from south India was found to be methyl salicylate chemotype. Hence, H. indicum could be a good source of methyl salicylate, which is the main constituent of the oil. In conclusion, the essential oil constituents vary in plant parts as well as in different localities growing plants. The plant T. rhomboidea is a high valued medicinal plant and as per the geo-climatic distribution of the flora, botanicals are consumed by the people as their own localities to cure the diseases. Secondary metabolites of the plants are changes in quantity or produced different chemotypes which may alter the biological activities. This study revealed that qualitative and quantitative difference in case of B. lanceolaria oil and compared from the previous reports, could exhibit altered bioactivities. Further studies are required to investigate the biological activities of the volatile constituents of these plants.