Taxonomía y sistemática

 

Geographic variation in Incilius occidentalis (Anura: Bufonidae), an endemic toad from Mexico, with a redescription of the species and delimitation of the type locality

 

Variación geográfica en Incilius occidentalis (Anura: Bufonidae), un sapo endémico de México, con una redescripción de la especie y delimitación de la localidad tipo

 

Georgina Santos-Barrera*

 

Departamento de Biología Evolutiva, Facultad de Ciencias, Universidad Nacional Autónoma de México. Circuito Exterior s/n, Ciudad Universitaria, 04510 México, D. F., Mexico. * gsantos@ecologia.unam.mx

 

Recibido: 19 septiembre 2013
Aceptado: 22 enero 2014

 

Abstract

A taxonomic revision of the populations referable to the wide ranging species Incilius occidentalis was conducted based on 10 morphometric variables and other external morphological traits. Thirty three populations were geographically defined from more than 220 locality records of the species. A brief summary of the nomenclatural history of this species is presented and the external morphological variation is analyzed. Incilius occidentalis is here redescribed on the basis of comparisons with the 3 specimens housed at the Old Collection of the Torino Museum, and over 850 specimens held at several herpetological collections. Statistical analysis using principal components analysis (PCA) demonstrated that SVL concentrates the main part of the variance observed in these populations. No distinguishable populations were detected on the basis of morphometric differences using the Tukey HSD analysis. Body proportions are fairly similar between the 33 populations defined, suggesting the idea that the typical body shape of the bufonid genera is also perceived in this species. The species I. occidentalis is fully redescribed, 1 lectotype and 2 paralectotypes are designated. The geographic range of this species is accurately defined and the type locality is restricted to the City of Guanajuato, and surroundings, Mexico.

Key words: amphibiofauna, morphometrics, taxonomy.

 

Resumen

En este trabajo se revisó la variación geográfica en la morfometría de las poblaciones que conforman la especie Incilius occidentalis, que se distribuye ampliamente en el noroeste y centro de México; se presenta además una revisión de la historia nomenclatural de esta especie. Para analizar la variación morfométrica se definieron 33 poblaciones provenientes de más de 220 localidades, midiéndose diez variables morfométricas estandar y otras de morfología externa en ejemplares adultos. El análisis de componentes principales (ACP) muestra que la variación observada se concentra en la variable LHC, sin embargo, la prueba de Tukey-HSD para analizar diferencias interpoblacionales mostró que no existen poblaciones morfométricamente distinguibles dentro de esta especie. La forma y el tamaño del cuerpo son similares entre las poblaciones, lo que sugiere la idea de que la forma típica que define a los bufónidos puede confirmarse en esta especie. I. occidentalis se redescribe mediante la comparación de los 3 ejemplares tipo depositados en la Colección Antigua de la Universidad de Turín, Italia y más de 850 ejemplares depositados en diferentes colecciones herpetológicas. Se designan un lectotipo y 2 paralectotipos, y se define la distribución geográfica de esta especie, restringiendo la localidad tipo en la ciudad de Guanajuato y alrededores.

Palabras clave: anfibiofauna, morfometría, taxonomía.

 

Introduction

The pine toad, Incilius occidentalis is a widespread endemic species from Mexico. It has been reported from more than 220 localities from west-central Durango to the south reaching the Isthmus of Tehuantepec on the Pacific versant, and from the Atlantic Versant of San Luis Potosi to the south reaching central Veracruz and eastern Oaxaca (Fig. 1). Although the species is commonly known as the pine toad, it can be found in a wide variety of habitats and microhabitats, such as oak and pine-oak forests, scrublands, arid and semi-arid areas and even cloud forest, at elevations from 150 to 2 600 m. Basic data from the first collections of this toad are unclear, however it can be speculated (from the collector's notes F. Craveri) that the 3 specimens in the type series were collected around mid XIX Century. These individuals are housed in the Old Collection of the Torino Museum in Italy. Being a wide ranging species, as well as an ecological generalist, a revision of the taxonomic situation of I. occidentalis was needed. The first approach to the taxonomic study of this species was the review of the nomenclatural history, and secondly the analysis of its geographic variation in morphology and morphometry.

Review of the nomenclatural and taxonomic history of Incilius occidentalis. The nomenclatural history of the toad I. occidentalis has been poorly discussed although several changes have occurred since its description. In the first half of the XIX century the Italian explorer F. Craveri, collected in Mexico 3 toads which he referred to the genus Bufo; he did not record the precise locality or the date of collection. These individuals were donated to the Herpetological Collection of the Torino Museum, Italy (MZUT) and catalogued as Bufo occidentalis by De Fillippi (presumably, the collection manager according to the annotations in the original catalogue). Around 1878, Lorenzo di Camerano, a naturalist present at the Torino Museum (MZUT) studied these 3 toads and referred to them, in print, as Bufo intermedius Günther, 1859. He presented a brief description of these individuals; however, he included the name Bufo occidentalis as a synonym of B. intermedius because he found the name in De Fillipi's annotations at the museum records (Camerano, 1879). For many years afterward, the name was largely ignored, with only occasional records cited from Mexico, all of them assigned to Bufo intermedius (e. g. Dugès, 1869). In Kellogg's classic paper on amphibians of Mexico at the United States National Museum (USNM), the name B. intermedius was considered a junior synonym of B. simus Schmidt, 1857, and consequently B. occidentalis became a synonym of B. simus (Kellogg, 1932). Kellogg did attempt to examine the 3 specimens from the Torino Museum, with no success; apparently the specimens were misplaced or temporally lost at the time of his visit. Concerning Bufo simus, this species was described by O. Schmidt (1857) from a series of 9 froglets collected by the botanist J. Von Warsewicz at the Chiriqui River, Bocas del Toro, Panamá. The validity of this species was questioned by Firschein (1950) who noted that the type locality and the localities known for Bufo simus in Mexico were remote and suggested that both populations should be considered as different taxa. Firschein (1950) also suggested that the collection data of B. simus could be erroneous since there was enough evidence that the celebrated collector J. Von Warszewicz was somewhat careless when documenting collection localities (see Savage, 1970 for other examples from reptiles and amphibian collections). On the basis of these uncertainties and the fact that the specimens collected by Warszewicz were juveniles, Firschein (1950) recommended the use of the name B. occidentalis Camerano, 1879 for all the specimens collected in México that had been assigned to B. simus Schmidt, 1857. Taylor (1951) supposed the rediscovery of this taxon since he examined an adult individual at the Museum of Zoology (UMMZ-58430) from Boquete, Chiriqui River Province, Panamá; he assigned this individual to Bufo simus, based on the absence of ostia pharyngea and the development of the supratympatic crests. Taylor discern from Firschein's (1950) position to consider Bufo simus as a nomem dubium. Savage (1972) examined the syntypes of B. simus housed at the British and Wien Museums, and noted that there was no morphological relationship between the specimens collected in Central America and those recorded from Mexico. He concluded that probably the syntypes of B. simus could represent the juvenile form of an unidentified species from South America. Recently, De la Riva (2004) made comparisons with other Bufo species from South America and concluded that B. simus is a synonym of B. spinulosus (later Rhinella spinulosa, Chaparro et al., 2007).

The other taxon related to the I. occidentalis history is Bufo monksiae Cope, 1879, a species almost never mentioned in the literature (Santos-Barrera, 1995). It was described based on an immature individual donated by Alfredo Dugés who collected the specimen in 1877 in Guanajuato, Mexico. The type specimen (USNM 9896) was examined by Kellogg (1932) who determined that it represented an immature individual of B. simus based of the presence of a mid-dorsal line and an interorbital transverse bar. These and other informative features such as the color of dorsal and ventral surfaces as well as the dorsal distribution of skin granules, and cranial crests are impossible to describe because of the poor state of preservation of the type specimen (Santos-Barrera, pers. obs). Bufo monksiae probably belongs to a population of I. occidentalis from central Mexico. In 2011, a new species was described from the I. occidentalis group, this species inhabits at northwestern Chihuahua and Durango, México and was discovered mainly on the basis of morphological and allozymic evidence (Santos-Barrera and Flores-Villela, 2011; Santos-Barrera et al., in prep.).

From a complete morphological inspection of the 3 specimens of Incilius occidentalis at the MZUT and the comparison with the extant populations of toads referable to Incilius (Bufo) occidentalis, it is evident that this species represents a complex of cryptic species whose alpha taxonomy is presented here. Previous works mentioned I. occidentalis as an inhabitant of most parts of western and central Mexico (Smith and Taylor, 1948). At this time, we know that I. occidentalis occurs in several localities at a great variety of habitats from intermediate to high elevations (Fig. 1), and for this reason several individuals of the genus Incilius have been erroneously assigned to I. occidentalis (Santos-Barrera, 1995). The goals of this study were: 1), to analyze the morphological and morphometric variation among populations of I. occidentalis in order to determine if this information can be useful to identify cryptic species; 2), to describe the geographic variation in morphometrics of this species considering the conservative pattern in morphology of toads, and 3), to redescribe the species based on the type series specimens in order to finally define the diagnostic characters that unmistakably lead to the correct identification of specimens and populations representing I. occidentalis.

 

Materials and methods

A total of 470 adult and 371 young individuals of Incilius (Bufo) occidentalis from 25 herpetological collections were examined (Appendix 1). According to the geographic location of the records 33 taxonomic populations were defined along the geographic range of the species (Fig. 1, Appendix 2). Ten standard morphometric variables were recorded for each individual in concordance with Mendelson (1998) and Mendelson et al. (2005): SVL (snout to vent length); HL (head length); HW (head width); TL (tibia length); longest diameter of the tympanum (TYMP); eyelid length (EYELID); parotoid length (PAROTOID); inter-orbital distance (IOD), the distance between the inner edge among both eyes; snout-nostril distance (SND), from tip of the snout to the anterior edge of the nostril; and eye-nostril distance (END), from the anterior border of the eye to the posterior edge of the nostril. Measurements were taken using a digital caliper (0.001 mm). All measurements were made by the same person (GSB) and always with the same caliper to avoid errors from different observers and instruments (Hayek et al., 2001). In addition 5 external morphological features were analyzed: 1), dorsal color pattern; 2), ventral color pattern; 3), shape and distribution of the granulation on the skin; 4), development and arrangement of the cranial crests, and 5), development of webbing of the foot (webbing formulae). Morphological terminology for tubercles on the fingers and toes follows that of Savage and Villa (1986), the webbing formulae is that defined by Savage and Heyer (1967), and Myers and Duellman (1982), and subsequently modified by Savage and Heyer (1997). Description of coloration of body surfaces follows Smithes's catalogue of colors (Smithe, 1975) (numbers in parenthesis). To test for sexual dimorphism an analysis of variance (ANOVA) was performed previous to start the geographic analysis. Based on these results, sexes were analyzed separately (results not shown). Adult males were defined by external inspection checking for the presence of vocal slits as well as nuptial pads in the first and second fingers, otherwise, a combination of general aspect, body size and lack of males features the individuals were considered as females. For an initial approach to the morphometrics of this species a principal components analyses (PCA) was performed with no transformed variables and based on covariance matrix. The PCA is a tool that standardizes and centralizes data, then the independent variables can be reduced to 1, 2, or more components where variation is concentrated or summarized, this is useful when variables are correlated, as is the case in this study (McKillup, 2012; Rencher, 2002). Scores from the 10 components for each individual were stored in the database and used to check for geographic variation by performing an ANOVA with populations as the independent variable. Only for this analysis, populations with less than 5 samples were excluded, causing the exclusion of 10 male populations (3, 7, 9, 10, 17, 18, 19, 21, 23 and 31) and 8 for females (7, 17, 18, 22, 23, 24, 29 and 30). According to the results of the ANOVA, for the variables where significant differences were revealed, a Tukey HSD test (honestly significant differenced) was applied. This test compares each group mean with every other group mean in a pairwise manner, populations differing from the groups designed with a letter can be considered as different (Quinn and Keough, 2003). All statistical analyses were performed with JMP Academic program (V. 10.0.0. Instituto de Ecología, UNAM) and based on the original, non-transformed values of the measurements for the reason that normality distribution of each variable was previously verified with the Shapiro-Wilk test (Tabachnick and Fidell, 1989; data not shown) and because magnitude orders of measurements are at the same rank.

 

Results

Morphometric analysis. The ANOVA across sexes revealed size differences between adult males and females in I. occidentalis (data not shown). Since all variables resulted significantly different, the statistical analyses were performed for each sex separately. The PCA revealed that variation in morphometrics of I. occidentalis is concentrated in 5 variables: SVL, TL, HW, HL, and PAROTOID, although the significance of each variable differs in each sex (Table 1). The first component (PC 1) accounts for 91.4% of the variation in males and 89.6% for females, the variable with the greatest contribution to this component is the SVL with scores of 0.85769 for males and 0.88375 for females, respectively. This is understandable since this variable represents the general body size, then, the second variable becomes more important to define differences between populations, this is TL for males (0.32686) and HW for females (0.27170); the third variable contributing to this component is HW for males (0.27121) and TL for females (0.27139). The fourth contribution is provided by HL for males (0.21591) and females (0.19593), the fifth contribution is the parotoid length with scores of 0.12600 for males, and 0.11558 for females. The rest of the variables have an insignificant contribution in this component (see Table 1). The second component (PC 2) accounts for 3.2 and 3.71 of the variance, in males and females. Scores from PCA indicate that TL and HW are the most important variables in males and females, respectively; however, SVL is negatively correlated with these variables, and in general, the contribution of this component is not comparable with PC 1. A graphic representation of the variables performance can be seen in figure 2. The 2 dimensions plot show the concentration of points, this means that the individuals sampled share a similar body size; longitude and direction of arrows indicate the value of each variable to explain the whole variance, it is evident the great importance of SVL in Component 1 in both sexes, although this variable is more conspicuous in males than in females, the same occurs with the variable TYMP. An interpretation of these results is that females are greater in size but also have a broader head whilst males seem to have a small body size with a small head too, however other variables related with head size as IOD, SND and END have an irrelevant role in the morphometrics of the species; the tympanum, when present, is more conspicuous in males (Fig. 2, see taxonomic comments). Results of the ANOVA across populations using the scores of the PCA show significant differences in the components 1, 3, 4, 6 and 10 in males (see table 2). Regarding females, differences arise in the components 1, 4, and 5 (Table 2). The Tukey HSD test applied to these components indicates that there are no distinguishable populations that can be separated on the basis of morphometric differences nor in males, neither in females (Appendix 3).

During the course of this project, while reviewing all populations referred to I. occidentalis, the population from Chihuahua was identified as a new species (Santos-Barrera and Flores-Villela, 2011). Two other populations were identified as different and will be considered later (Santos-Barrera and Flores-Villela, in prep.). The remaining populations share the same morphological external design; all these populations are recognized as Incilius occidentalis, the redescription is presented below.

Incilius occidentalis Camerano, 1879

Bufo intermedius Günther, Catalogue of the Batrachia Salientia in the Collection of the British Museum. pp. 1859.

Bufo occidentalis Camerano, Atti. Accad. Sci. Torino, 14: 886-889, 1879.

Bufo intermedius Dugès, La Naturaleza, 1: 137-145. 1869.

Bufo monksiae Cope, Proc. Amer. Philos. Soc., 18: 263, 1879.

Bufo simus Schmidt, Denkschr. Akad. Wiss. Wien. math-nat., 14: 254-255, 1857; Kellogg, Bull. U. S. Nat. Mus. 160: 1-224, 1932. Smith and Taylor, 1948. U.S. Natl. Mus. Bull (194):42-43.

Cranopsis occidentalis Frost, Grant, Faivovich, Bain, Haas, Haddad, de Sá, Channing, Wilkinson, Donnellan, Raxworthy, Campbell, Blotto, Moler, Drewes, Nussbaum, Lynch, Green, and Wheeler, 2006, Bull. Am. Mus. Nat. Hist., 297: 364.

Ollotis occidentalis, Frost, Grant, and Mendelson, 2006, Copeia, 2006: 558. By implication

Incilius occidentalis, Frost, Mendelson, and Pramuk, 2009. Copeia, 2: 418; Frost, 2008, Amphibian Species of the World Online, vers. 5.4: Required change due to seniority of Incilius over Ollotis.

Lectotype: MZUT An 210-2. An adult male from México collected by Federico Craveri. Locality and date of collection unknown.

Paralectotypes: MZUT An 210-1 and MZUT An 210-3. Both males from Mexico, collected by Federico Craveri. Collection locality and date unknown too. See below type locality.

Diagnosis. Incilius occidentalis can be recognized by the following combination of characters: 1), medium to large-sized individuals (SVL range, males: 52.37-84.15; females: 76.42-91.93); 2), snout truncate in profile; 3), adult individuals with weakly developed cranial crests; 4), supraocular crest most developed, postocular crest evident in large individuals, parietal crest only evident in the largest individuals; 5), parotoid gland elliptical, medium sized (about 51% of HL), separated from eyelid by short series of granules; 6), tympanum conspicuous, round; 7), vocal slits in males unilateral or bilateral; 8), dorsal surfaces of adults covered with numerous medium-sized sharply pointed granules with keratinized tips, dorsal surface of juveniles smooth; 9), lateral descending line of tubercles absent; 10), dorsal coloration consisting of olive brown background with distinct pale cream vertebral stripe defining 2 dark brown lateral blotches, vertebral line interrupted at mid body in many individuals, and 11), ventral surfaces cream or pale yellow with no marks except in young individuals where pale gray dots may be present (Fig. 3).

Comparisons with sympatric and other related Bufonid species. Incilius occidentalis can be distinguished from sympatric species of the genus Anaxyrus by the dorsal color pattern, which consists of a medium light brown line often interrupted and bordered by lateral continuous olive to dark brown irregular stripes (versus numerous dark grey-green blotches on a light grey background in Anaxyrus cognatus, and numerous small brown dots on a light grey to brown background in A. compactilis). The body size of adults is noticeably larger in A. cognatus, males of Incilius occidentalis reaching 87 mm in SVL (versus 115 mm in A. cognatus). I. occidentalis can be distinguished from A. compactilis by of the soft texture of the dorsal skin which is covered with medium sized granules, smaller and more abundant at the flanks and limbs (uniformly rugose skin because of the presence of abundant small pointed granules). From A. mexicanus, because of the development of the cephalic crests, well developed supraoculars, postocular reduced and inconspicuous parietals in I. occidentalis (versus supraoculars less developed and parietal often lacking in A. mexicanus). Dorsal color pattern is reddish brown with scattered small dark brown dots sometimes suffused to form blotches in A. mexicanus. From A. punctatus differs because of the size and shape of the parotoid glands, elliptical and well developed in I. occidentalis (versus small and rounded). The color pattern of A. punctatus is highly variable, presenting a light grey to olive or reddish brown background with numerous red to brown dots almost always on a black ring at the base. Other Incilius species are not sympatric with I. occidentalis. In the phylogenies of North American Incilius and Anaxyrus species by Pauly et al (2004) and Bufonidae by Van Bocxlaer et al. (2010), I. occidentalis is the sister species of I. alvarius. I. occidentalis can be separated from this last species by differences of dorsal color pattern which consist of dull olive to gray ground without lateral blotches and scattered marks in I. alvarius, this pattern contrasts with the brown pattern of blotches of I. occidentalis described above. In addition, I. alvarius exhibits dorsal smooth skin with conspicuous enlarged glands on the thighs (Oliver-López, 2009), both features not present in I. occidentalis. Pyron and Wiens (2011) relate I. occidentalis as the sister taxon of I. tacanensis, this last species differs from I. occidentalis mainly because the color pattern and granulation of dorsal surfaces, including dark brown bands bordered by lighter lines with a light mid-dorsal line in I. tacanensis, characters not present in I. occidentalis, moreover, a line of small conic granules runs along the flanks in I. tacanensis.

Description of the lectotype. An adult male with small body, SVL 56.92 mm. The head is truncate in lateral view and almost triangular in dorsal view; supraocular crest distinct, postocular and parietal crests less conspicuous; parotoid gland ovoid, length 55.62% of HL; tympanum distinct, nearly round, 30.67% of the diameter of the orbit; dorsal surfaces are covered with abundant large rounded granules with numerous pores, keratinized spines on the tip are severely damaged because of the storage and time of preservation; granules on the ventral surface uniformly distributed including the inguinal area; forelimbs short; fingers slender, webbing absent; internal palmar tubercle is rounded with dark brown pigmentation; external palmar tubercle is piriform, larger than internal palmar tubercle; relative length of fingers 3>1>4>2; nuptial excrescences on fingers 1-3, conspicuous, dark brown; subarticular tubercles distinct, paired on the first finger; supernumerary tubercles paired on the third and fourth digits; hind limbs are slender; length of toes in decreasing order is 4, 3, 5, 2, 1; webbing formulae, right foot I1-1II1-1III1 ¹/₂-3IV3-1V, left foot I 1-1 II 1-2 III 2-3 IV 3-2 V. Short rows of keratinized tubercles on lateral surfaces of tarsi of feet; internal metatarsal tubercle large, 2X larger than the external metatarsal tubercle. Vocal slits bilateral.

Coloration in preservative. Background of the dorsum of body, including head and limbs, pale olive (olive gray, 42) with a distinctive pale mid dorsal stripe forming 2 paravertebral dark brown bands (olive brown, 28). There are 2 transverse parallel brown stripes on each side of the head. Lateral surfaces with several irregular small blotches. Parotoid glands are the same color as the rest of the dorsum with darker markings. Dorsal surfaces of hind limbs with transverse brown stripes. Ventral surfaces are cream and immaculate. The juvenile individuals have small black or dark gray markings on the throat and belly. Ventrally, fore limbs and thighs are immaculate.

Coloration in life. Adult specimens of I. occidentalis have a dorsal color pattern that varies gradually between individuals, with olive or unmarked brown being the most common background color (colors 42 and 44), and with a distinct pale mid-dorsal stripe forming two paravertebral dark olive to brown bands (colors 28 and 129); the vertebral stripe is often interrupted at mid body or below. Ventral surfaces are generally cream color (54) to light yellow with no marks.

Variation. Background color and lateral blotches on the dorsum can be darker in some individuals, especially the younger ones. The granulation of dorsal skin surfaces is clearly pronounced in juveniles, less abundant and sharply reduced in old individuals. Both paralectotypes resemble lectotype in dorsal and ventral color patterns. Webbing formulae of the hind feet can slightly vary, especially in relation to fingers 3, 4 and 5, but no differences exist between populations or through sexes. Variation in size of the body across sexes is remarkable, the largest average in SVL recorded in males is population 31, central Veracruz (82.32) and the lowest population 23, southeastern Oaxaca (53.84). Regarding females, the highest SVL recorded is population 16, Michoacán center (88.03), and the lowest from population 11, lowlands of Guerrero (66.85). During field work in Aguascalientes, a large female reaching SVL of 112 mm was collected; this individual was eliminated from the dataset to avoid biases in the statistical analyses.

Tadpoles. The tadpole has not been described.

Distribution, ecology and conservation. I. occidentalis occurs in northern and central Mexico from the highland Plateau in the states of Durango, Zacatecas, and Aguascalientes to the south and west through east Jalisco and Colima, to Central Mexico along Querétaro and northeastern Guanajuato, south and westward to Michoacán, Guerrero and central Puebla, to the east reaching central Veracruz and northern Oaxaca (Fig. 1). This species occurs in a great variety of environments, however it seems to prefer arid and semiarid scrub, mainly composed of mesquite (Prosopis spp.), huizache (Acacia spp.), and Opuntia (Zamudio et al., 1992). In some elevated areas above 1 000 m at the states of Durango, Guanajuato and Oaxaca it occupies pine-oak and oak forests (pers. obs.). It is possible to find individuals of this species in both pristine and moderately disturbed areas, often maintaining large populations in areas not too proximal to human settlements (Santos-Barrera and Urbina-Cardona, 2011). All observations suggest that this is a nocturnal species, avoiding high daytime temperatures by sheltering beneath rocks and logs or even under the ground. During the night, it is possible to find adult individuals adjacent to permanent or temporary springs and low rivers, commonly hidden beneath the shrubs and in the roots; it seems to avoid permanent ponds and pools. This is a stream dwelling species, in the rainy season (July to September) in northern Mexico it is possible to observe aggregations of toads along the rivers shores. In the arid and semi-arid areas of Puebla, adult individuals may be active until November and early December. Diet of adult individuals mainly consists of ants, orthopterans and several other insect larvae, according to observations of stomach contents (Santos-Barrera, unpublished data). This species is classified as Least Concern in the Red List of the IUCN (IUCN, 2012), and it does not appear in any other conservation listings. However, although it is considered as a widespread species, numerous populations along its range have experienced continuous declines, almost always due to habitat loss and persistent disturbance of native forested areas for logging, to open areas for cultivation and human settlements, this has been especially observed in central Mexico (Santos-Barrera, pers. obs).

Taxonomic comments. According to Taylor (1951), one important character to separate the species Bufo simus, from Bufo monksiae was the absence of the ostia pharyngea as well as the hidden tympanum. Smith (1952) also mentioned the presence of this character when relates Bufo tacanensis with B. occidentalis since both species examined by him have pharyngeal ostia. However B. occidentalis presents a hidden tympanum while B. tacanensis lacks this feature. Smith (1952) did not mention the locality of the B. occidentalis examined to compare with B. tacanensis. The ostia pharyngea, was not examined in this study, and regarding the presence and size of the tympanum, this feature seems to be an uninformative character because it might be or not evident in this species. The only populations that consistently lack tympanum are 10 and 11, the Guerrero highlands and Guerrero lowlands, respectively. It was also noted that males may have 1 or 2 vocal slits without a discernible pattern of distribution of this character. The type locality of this species was not specified by the collector F. Craveri or the author Camerano (1879), based on the comparisons of numerous individuals with the type specimens, that clearly coincide with central Mexico morphotypes, and based on the speculated route of collector Craveri (Gavetti, pers. comm), it is recommended to follow Smith and Taylor (1948) criterion to define the type locality to Guanajuato city and surroundings.

 

Discussion

The use of external morphological characters and morphometrics is a useful tool to describe and identify species (Mendelson et al., 2012). Even more, these features can be related with environmental data to search for evolutionary patterns and life history traits and to explore into phylogenetics (Rosso et al., 2004; Zelditch et al., 2000). In the present study, morphology and morphometrics have contributed to nearly complete the resolution of the long-standing taxonomic enigma of the I. occidentalis complex thus completing the Alpha taxonomy. The study of morphological features in I. occidentalis lead to the recognition of the population of Chihuahua as a different species, but the morphometric structure in this last species has almost the same body proportions of the rest of the I. occidentalis populations. In terms of morphometrics, it is evident that only small differences exist among populations of I. occidentalis along its geographic range. These differences are patent mainly in males, as have been noticed in other Incilius species (Mendelson, 1998; Mulcahy and Mendelson, 2000; Mendelson et al., 2005). In this study, the largest male individuals were identified in southern Mexico, (population 31, southern Veracruz) averaging 82.32 of SVL, and 88.03 (population 16, central Michoacán), hence, largest males do not correspond to largest female populations. Neither small size populations have coincidence (Fig. 1).

Morphology and morphometrics in bufonid toads can be useful to make interspecific comparisons, or to check for variation into highly variable intraspecific species (Mendelson et al., 2011). Revising the global results of this study it is possible to conclude that there is not an identifiable geographic morphometric pattern among the populations of this species studied, as it has been described in other lowland Bufonid species (Mulcahy et al., 2006). These results could be the consequence of the generalist habits of I. occidentalis that can occur in a great variety of environments, (see the natural history section), at a wide elevation range from 200 m to 2500 m, dwelling almost always on the ground, beneath rocks and logs and/or burrowing in holes in the ground. At the present time, none of the known populations can be separated only on the basis of morphometric variables, which is not surprising given the conservative body shape documented in many bufonid species (Maxson, 1984; Mendelson et al., 2011). In fact, the family Bufonidae is complex, with a superficial similarity. This conservative morphological pattern in concordance with some ecological traits could promote their diversification and expansion from the South American morphotype (VanBocxlaer et al., 2010; Zug, 1993).

The PCA was selected as the first approach to explore the morphometric data of I. occidentalis because this analysis constructs new variables that better resume the observed variation (McKillup, 2012), which was particularly helpful in this study. It is well known that body proportions in anurans reflect 4 basic locomotor categories (Pough et al., 1998). The PCA demonstrated that both, males and females of I. occidentalis retain the standard anuran body shape that fits well into the walker-hopper type, with short forelimbs and medium-long hindlimbs (Pough et al., 1998). By examining carefully plots of PC 1 vs. PC 2 for males and females (Fig. 2), it is evident that populations form just one group, no outliers or dispersed points can be seen, thus forming a narrow ellipse where points are concentrated. The PCA showed that the general measurement of body, expressed as SVL explain the majority of the variation observed, this could be the cause of this concentration of points, other variables as HW, HL, and TL are highly positively correlated with SVL, then, a large snout to vent length is associated to a long head or to a long tibia. This was expected to some extent, since allometric effects were controlled by excluding juveniles or immature individuals. Commonly, in a PCA the first component explain 70 to 75% of the variance, and the second and third the 15 and 5% respectively (McKillup, 2012). In this study, for males, the PC 1 explains 91.4 % of the variance and the second just 3.2%; this is an unusual situation that can be explained by the conservative morphological pattern of Bufonidae mentioned above, this determines an isometric type that is conspicuous in both, males and females. The comparisons with the Tukey HSD test showed that all populations can be grouped by morphometric differences in no more than 4 groups, thus confirming a stable morphometric design (Appendix 3). This result has been recognized in other anuran species where relative proportions of the body remain stable even when an increase in body size and sexual dimorphism is detected (Duellman and Trueb, 1990; Mendelson et al., 2005).

The clarification of I. occidentalis systematics is in progress, by the moment, it can be concluded that I. occidentalis is a widespread species. Variation in the morphology is not reflecting taxonomic variation in the group. It is impossible to define a clear tendency in color pattern of dorsal and ventral surfaces; this is the same situation for distribution of granulation and development of cranial crests. Morphological features examined are insufficient to define species limits, besides the ones already recognized. Low levels of variation persist in characters such as coloration of dorsal and ventral surfaces. No consistent variation exists in the development of cranial crests and parotoid shape and size. The taxonomic importance of the toes and fingers size and development of webbing is so reduced that their information was used only for descriptive purposes. The rest of the variables show similar patterns, just few individuals are variable, even in the same population; other differences between individuals can be attributed only to age and development with no clear geographic trend, as is commonly observed in other bufonid toads (Mendelson et al., 2011). This is also the case of the tympanum; this structure can be evident or non-evident in individuals of the same population regardless of sex. However, measurement of tympanum was evaluated in all populations, except of populations 10 and 11 from Guerrero where individuals of both sexes consistently lack this character. Other relevant data that should be studied in this species complex are those related to natural history, including tadpole descriptions, call variation and habits as well as biogeography and evolutionary history. Knowledge of the phylogenetic relationships and species limits in the I. occidentalis complex is still in progress (Santos-Barrera and Flores-Villela. in prep). Different molecular studies recovered I. occidentalis and I. alvarius as sister taxa, this last species is an inhabitant of the Sonoran desert and recently recorded in Chihuahua, northern Mexico, thus controversially relating two geographic and morphological dissimilar species. The complete picture of the phylogeny and biogeography in I. occidentalis can certainly clarify this and other evolutionary questions (Pauly et al., 2005; Mendelson et al., 2011; VanBocxlaer et al., 2010).

 

Acknowledgements

I want to thank Dr. Elena Gavetti and Dr. Franco Andreone from the Museo Regionali di Scienzi Naturali, University of Turin (MZUT), for the loan of the type specimens of I. occidentalis. Thanks also to the curators of the following Museums who kindly provided the scientific material: AMNH (American Museum of Natural History), BYU (Brigham Young University, Monte L. Beam Museum), EBUAP (Escuela de Biología, Universidad Autónoma de Puebla), MZFC (Museo de Zoología, Facultad de Ciencias, UNAM), IBH (Instituto de Biología-Herpetología), TCWC (Texas Cooperative Wildlife Collection), ENCB (Escuela Nacional de Ciencias Biológicas-IPN), (UTA), University of Texas at Arlington, (UIMNH), University of Illinois Museum of Natural History (INHS), Illinois Natural History Survey. For field assistance I want to thank Luis Canseco, Francisco Vargas, Alberto Mendoza and Gustavo Fernández. M. J. Hernández, Juan Fornoni and Armando Martínez advised with the statistical analyses. Heliot Zarza and E. Saturno helped with preparation of figures and tables. Unlimited support in many ways was provided by Oscar Flores-Villela. Financial funding for this project was provided by Conabio Project L244 (GSB and OFV) and two fellowships to GSB, DGEP-UNAM and AMNH, New York.

 

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