Abstract

TOLEDO SALAS, Analí et al. EVALUATION OF THE CADMIUM EFFECT ON PHENOLOXIDASES FROM Epithelantha micromeris CALLUS TISSUE, INOCULATED WITH RHIZOBACTERIA. Rev. Int. Contam. Ambient [online]. 2018, vol.34, n.4, pp.725-735. ISSN 0188-4999.  https://doi.org/10.20937/rica.2018.34.04.13.

A biotization process is proposed as a new technique in plant tissue culture that allows in vitro the association between plants and microorganisms. Bacterization is the inoculation of callus tissue cultivated in vitro. In this process, bacteria promote the plants growth, induce changes in their development and increase the response mechanisms against stress conditions. One of the most notable effects of plants exposed to cadmium is the increment or inhibition of antioxidant enzymes, with the possible participation of phenoloxidases. The present study evaluated the response of callus tissue of Epithelantha micromeris (Engelm.) inoculated with plant growth promoting rhizobacteria through guaiacol peroxidases and hemoperoxidases activity as oxidative stress responses produced by cadmium exposition. From the results obtained, rhizobacteria occurrence per se generated a stress condition; however, in the presence of cadmium an antioxidant response was induced that includes the increment of phenoloxidases, which are oxidative stress indicators.

Keywords : heavy metals; in vitro culture; peroxidases; hemoperoxidases; antioxidant response.