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Gaceta médica de México
On-line version ISSN 2696-1288Print version ISSN 0016-3813
Abstract
CAMACHO-ORTIZ, Adrián et al. Azithromycin effect on multidrug-resistant Acinetobacter baumannii biofilm production and composition. Gac. Méd. Méx [online]. 2021, vol.157, n.5, pp.497-502. Epub Dec 13, 2021. ISSN 2696-1288. https://doi.org/10.24875/gmm.21000380.
Objectives:
To evaluate the effect of azithromycin (AZM) on biofilm formation and composition in multidrug resistant (MDR) Acinetobacter baumannii.
Material and methods:
Ninety-six A. baumannii isolates were studied. Antimicrobial susceptibility and sub-minimum inhibitory concentration (sub-MIC) were determined by the broth microdilution method. Carbapenemase genes were detected by polymerase chain reaction and clonal diversity by pulsed-field gel electrophoresis (PFGE). Biofilm formation without AZM and AZM sub-MIC were determined by crystal violet staining. AZM-free biofilm composition and AZM sub-MIC were determined by detachment assays.
Results:
The selected A. baumannii were MDR; 93.8 % were carbapenem-resistant and 24 were OXA-24-positive. PFGE showed predominance of clones A (53 %), B (34.7 %) and C (12.5 %). Biofilm production at AZM sub-MICs decreased in 53.1 %, increased in 34.7 % and showed no differences in 12.5 % of isolates, in comparison with biofilm production without AZM.
Conclusion:
AZM sub-MIC can reduce biofilm production in A. baumannii MDR isolates with decreased protein and DNA in the biofilm. Our results may be useful in synergy studies for new therapeutic alternatives.
Keywords : Acinetobacter baumannii; Biofilm; Sub-MIC; Biofilm composition.