Abstract

GARCIA GONZALEZ, Rafael; BASILIO HERNANDEZ, David  and  HERNANDEZ RAMIREZ, Aurora. Parvovirus B19: Laboratory diagnosis. Rev. Fac. Med. (Méx.) [online]. 2012, vol.55, n.2, pp.4-10. ISSN 2448-4865.

Parvovirus B19 (B19) is the only known human pathogenic parvovirus. The spectrum of illness to which B19 is associated includes a wide range of diseases: erythema infectiosum, thrombocytopenia or granulocytopenia, aplastic crisis or hemolytic anemia in immunocompromised patients. The laboratory diagnosis of Parvovirus B19 infection is primarily confirmed by the detection of IgM antiviral antibodies using the ELISA technique, which is directed against both conformational and linear epitopes of VP1 and VP2 capsid. When IgM response declines, an IgG immune-response against structural proteins VP1 and VP2 becomes prominent and can detected by immunofluorescence, ELISA and Western blot. Polymerase chain reaction is now used as a routine analysis as well as complement or alternative to serology.

Keywords : Human parvovirus B19; diagnosis; IgM; IgG; ELISA; PCR.