Abstract

RIQUELME, B; RUBIO, O  and  VALVERDE, J. Optical sensor for the quantification of monoclonal antibodies. Rev. mex. fis. [online]. 2006, vol.52, suppl.2, pp.68-71. ISSN 0035-001X.

An optical sensor for the quantification of a monoclonal antibody (MoAb) in solution by the reflectometry technique is shown in this work. A silicon wafer was chosen as a reflecting surface. An amount of AB blood group antibody (anti-AB) is added to the sample when the pseudo-Brewster angle of incidence has been fixed. The reflected laser intensity is registered in real time as the protein is being adsorbed onto the wafer. The mathematical analysis of the results verifies that the antibody adsorption follows Langmuir's kinetics. From the curve analysis, the parameters related to the anti-AB concentration are extracted and the calibration curve is constructed. This curve allows us to obtain the desired commercial antiserum quantification.

Keywords : Laser reflectometry; immunosensor; Langmuir adsorption.