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Salud Pública de México
Print version ISSN 0036-3634
Abstract
ULLOA-GARCIA, Armando et al. Detection of Rickettsia typhi in Rhipicephalus sanguineus s.l. and Amblyomma mixtum in South Mexico. Salud pública Méx [online]. 2020, vol.62, n.4, pp.358-363. Epub May 16, 2022. ISSN 0036-3634. https://doi.org/10.21149/10160.
Objective:
To determine the presence of Rickettsia typhi in Rhipicephalus sanguineuss.l. and Amblyomma mixtum in southern Mexico.
Materials and methods:
Ticks were collected in humans and domestic animals. The presence of Rickettsia was determined by PCR and sequencing.
Results:
10/39 work vials amplified fragments of the gltA,htrA and ompB genes. On 7/10 from Rh. sanguineus s.l collected from dogs and in 3/10 of A. mixtum collected from horse and human. Sequencing indicated R. typhi in Rh. Sanguineus and A. mixtum with 100% homology (LS992663.1) for a region of the htrA gene and 99% (LS992663.1) with the regions of the gltA and OmpB genes. The minimum infection rate (TMI) for R. typhi was 3.88.
Conclusions:
Rhipicephalus sanguineuss.l. and Amblyomma mixtum are naturally infected with R. typhi in Southern Mexico.
Keywords : ticks; Rickettsia typhi; Mexico.