Abstract

PEREA SOTO, José Misael et al. Identification of Races and Biovars of Ralstonia solanacearum Isolated From Tomato Plants. Rev. mex. fitopatol [online]. 2011, vol.29, n.2, pp.98-108. ISSN 2007-8080.

Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases in economically important crops such as tomatoes, potatoes, eggplant, pepper and tobacco. The objective of this study was to identify strains of R. solanacearum isolated from tomato plants grown in Culiacan Valley, San Quintin, B.C. and Jalisco in Mexico to race and biovar levels. Eight strains of R. solanacearum from wilted tomato plants were isolated. Isolations were made on SMSA culture medium, and identified as R. solanacearum using morphological, physiological and biochemical studies, immunoassay strip and polymerase chain reaction (PCR) by using the universal primers 759/760 to amplify a 280 bp fragment from the ribosomal DNA. Race identification was performed with R. solanacearum race 3 specific primers. Based on the ability of isolates to produce acid from a panel of disacarides and sugar alcohols, all isolates belong to biovar 2 (Bv2).

Keywords : Bacterial wilt; tomato; Ralstonia solanacearum; Race 3; Biovar 2.