Abstract

GARCIA-NUNEZ, Hilda Guadalupe et al. Morphological and molecular characterization of native isolates of Trichoderma and its potential biocontrol against Phytophthora infestans. Rev. mex. fitopatol [online]. 2017, vol.35, n.1, pp.58-79. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1605-4.

This paper presents the morphological and molecular characterization of 10 native isolates of Trichoderma (TL2, TL4, TL5, TL6, TX7, TX8, TT6, TF8, TF10 and TJ6) and their phylogenetic relation, as well as its biocontrol ability against Phytophthora infestans. The results of the molecular characterization by amplifying the ITS1ITS4 region of nucle1 and TJ6). The phylogenetic analysis by maximum parsimony showed a close relation among the isolates of these two groups. Biocontrol tests between the antagonist and the pathogen showed significant differences (p<0.05). Strain TX8 had the highest inhibition percentage (98 %), while TL4 recorded the lowest percentage (49 %). The isolates of T. harzianum (TL5, TL6, TF10 y TJ6) showed percentages between 5565 %.

Keywords : T. asperellum; T. harzianum; antagonism; potato; Solanum tuberosum.