Abstract

ORTEGA-ACOSTA, Candelario et al. Evaluation of seed transmission of begomoviruses in roselle and roselle-associated weeds. Rev. mex. fitopatol [online]. 2019, vol.37, n.1, pp.135-146.  Epub Aug 21, 2020. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1806-3.

In the state of Guerrero, Mexico, the begomoviruses Okra yellow mosaic Mexico virus (OYMMV) and Whitefly-associated begomovirus 3 (WfaBV3) have been found in association with roselle (Hibiscus sabdariffa L.). The objective of this study was to determine if OYMMV and WfaBV3 can infect weeds commonly found in roselle commercial crops and if they are seed-transmitted. Roselle plants and weeds showing yellowing and mosaic were collected in Guerrero in 2016. Using universal primers for begomoviruses and specific primers for the 18s rRNA gene as an internal control, total DNA was analyzed by PCR. Begomovirus positive samples were later analyzed with primers specific for OYMMV and WfaBV3. One hundred of the roselle and weed seeds collected from symptomatic plants was analyzed for begomoviruses by PCR; 100 seeds from each roselle cultivar and 40 seeds from each weed were planted in seedling beds. Young leaves of roselle seedlings were analyzed by PCR after circular DNA amplification by the Rolling Circle. The WfaBV3 and OYMMV were detected in roselle mature plants and in the weed species Sida collina, S. aggregata, S. acuta, S. haenkeana and Malacra fasciata, OYMMV was detected in mature plants, in seeds and in seedlings. This is the first report of four new hosts for OYMMV and its transmission by seed.

Keywords : Hibiscus sabdariffa; Okra yellow mosaic Mexico virus; Whitefly-associated begomovirus 3; Rolling Circle Amplification.

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