Abstract

ORTEGA-ACOSTA, Candelario; OCHOA-MARTINEZ, Daniel Leobardo; ROJAS-MARTINEZ, Reyna Isabel  and  GUTIERREZ-GALLEGOS, Jorge A.. Phyllody of daisy (Dimorphotheca sinuata) associated to Candidatus phytoplasma asteris. Rev. mex. fitopatol [online]. 2019, vol.37, n.3, pp.444-453.  Epub Sep 30, 2020. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1905-3.

In Montecillo Texcoco, State of Mexico we observed daisy plants (Dimorphotheca sinuata) showing symptoms of phyllody, virescence and proliferation of axillary branches, all those induced by phytoplasmas. Therefore, the objective of this investigation was to detect the phytoplasma associated with these symptoms by PCR with primers P1/P7 and R16F2n / R2 (nested PCR). The 1200 bp PCR product was obtained from the PCR and it was digested with the restriction enzymes MseI (Tru91), AluI, Kpn1 and HaIII and restriction maps showed that the phytoplasma present in Dimorphoteca sinuata is Candidatus phytoplasma asteris (16Sr1-B). The sequences were deposited on the NCBI data base and had a similarity of 99% with Candidatus phytoplasma asteris group 16Sr1-B from Iran (MH638316.1). A phylogenetic analysis was performed with the Neighbour-Joining method in which the phytoplasm from daisy was grouped with Candidatus phytoplasma asteris. According to the symptoms observed in the field, analysis of restriction patterns, sequencing and phylogeny, the phyllody of daisy is associated with Candidatus phytoplasma asteris, phylogenetically related to the 16Sr1-B group.

Keywords : Floral reversion; RFLP; sequencing; phytoplasms.