Abstract

MORALES-MORA, Luis Angel et al. Characterization of strawberry associated fungi and in vitro antagonistic effect of Trichoderma harzianum. Rev. mex. fitopatol [online]. 2020, vol.38, n.3, pp.434-449.  Epub Nov 27, 2020. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.2005-7.

Mexico is the third largest strawberry producer in the world, where the production of this crop is of economic importance and generates foreign exchange in the country. The objective of the present investigation was to identify and morphologically characterize the fungi associated with a commercial strawberry field, as well as to determine the in vitro antagonistic capacity of the T-H4 strain of Trichoderma harzianum in isolated fungi. Samples of plants from the strawberry crop with fungal symptoms were collected, sown in PDA medium and monosporic cultures were generated for their morphological characterization. The identified fungi and the strain T-H4 were compared in a dual way. Were identified and characterized three fungi associated with the fruit (A. niger, Colletotrichum sp. and R. stolonifer), three on leaves and stem (Pestalotiopsis sp., Curvularia sp. and Alternaria sp.) and two associated fungi on the root (Rhizoctonia sp. and Fusarium sp.). The T-H4 strain presented an antagonistic level suitable for Colletotrichum sp., Pestalotiopsis sp., Alternaria sp., Rhizoctonia sp. and Curvularia sp., in vitro. It is suggested to carry out biological control evaluations with these isolates in the greenhouse and in the open field, as well as to determine their pathogenicity.

Keywords : Antagonist; taxonomic identification; mycelial growth; inhibition..