Abstract

GAITAN-HERNANDEZ, Rigoberto  and  BAEZ RODRIGUEZ, Iván. Mycelial growth of Lepista nuda native wild strains on culture media with different organic supplements. Rev. Mex. Mic [online]. 2008, vol.26, pp.41-49. ISSN 0187-3180.

Mycelial growth of four strains of the wood blewit mushroom Lepista nuda on culture media with different organic supplements (coconut bagasse, sugarcane bagasse, oak sawdust), pH (6, 7, 8) and temperature (20°C, 25°C) was evaluated. Linear growth rate (K_t) on agar medium was measured. Biomass production of mycelia and phenolic content on oak liquid culture, under two different gaseous interchange treatments, culture bottles with filter (F) and culture bottles without filter (WF), also were observed. Results showed a significant influence of the supplements and strains on agar. The best growth responses were observed on oak agar medium at 20°C and 25°C (0.417 and 0.410 mm d^-1, respectively). For both temperatures, in all treatments no differences significantly were observed. The highest K_t by L. nuda strains was registered at pH 7. On liquid culture, the F treatment significantly increased the biomass production (4.10 mg mL^-1) by almost 1.8 times compared to the WF treatment (2.29 mg mL-1) at 13 days of incubation. Phenols concentration decreased in both treatments but in F treatment the phenols concentration (0.79 mMol L^-1) decreased less than WF (0.62 mMol L^-1). No correlation between the biomass production and phenolic content was observed.

Keywords : Blewit; in vitro evaluation; coconut bagasse; sugarcane bagasse; oak powder.