Abstract

MEDINA GONZALEZ, Guadalupe Estefanía et al. Use of native Basidiomycetes in the biotransformation of buffel grass ( Cenchrus ciliaris ) to improve the nutritional quality. Rev. Mex. Mic [online]. 2016, vol.43, pp.31-35. ISSN 0187-3180.

The aim of the present work was to evaluate the capability of three native basidiomycetes from the Northeast region of Mexico to produce enzymes able to break down the main components of plant cell wall and their effect on the biotransformation of buffel grass (Cenchrus ci liaris) in order to increase its nutritional quality. Lignocellulolytic potential was evaluated on solid media and the biotransformation of buffel grass slabs was performed. Titers of cellulases, xylanases, and laccases were determined, as well as the fiber and protein content in the slabs. The basidiomycete reduced neutral detergent fiber (NDF) content on treated slabs. Trametes maxima CU1 showed the greatest lignolytic capability reducing 14.24% the lignin content of grass. Pycnoporus sanguineus CS2 and T. hirsuta CS5 reduced the content of cellulose (21.6 and 19.8% respectively), and hemicellulose (17.1 and 14.2% respectively) in comparison to the control. Treatment with fungi P. sanguineus CS2 and T. hirsuta CS5 increased in 31% the protein content of grass. These results denote that basidiomycetes increased nutrients bioavailability of buffel grass, hence increasing its nutritional quality.

Keywords : cellulases; laccases; Trametes spp.; xylanases.