Abstract

SOLIS-DOMINGUEZ, F. A et al. Towards the Monoxenic Culture of Glomus claroideum in Transformed Roots of Carrot. Terra Latinoam [online]. 2009, vol.27, n.1, pp.35-41. ISSN 2395-8030.

Arbuscular mycorrhizal fungi (AMF) are rhizospheric microorganisms that complete their life cycle only when they colonize the root system of susceptible hosts. The plant tissue culture accompanied by genetic transformation of roots has allowed the in vitro propagation of some AMF species. The objective of this investigation was to establish and to study the behavior of Glomus claroideum in monoxenic culture of transformed carrot roots by Agrobacterium rhizogenes. Although G. claroideum did not show intraradical colonization, the curly external mycelia had limited distribution and formed an entangled mass of hyphae, and some immature spores were observed. In some regions, the hyphae presented wall elongations forming crest-like structures, resembling those contact structures exhibited by some mycoparasitic fungi. The viability of the extraradical mycelia, estimated via the fungal alkaline phosphatase vital stain, was maintained after three months of the monoxenic culture. The possible causes of the behavior of G. claroideum under monoxenic culture are discussed.

Keywords : arbuscular mycorrhizal fungi; extraradical mycelium; in vitro culture.

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