Abstract

BAZAN LUGO, Eduardo; ALVAREZ CARDENAS, Cristian G.  and  TOTOSAUS S., Alfonso. Effect of physical pretreatment of wheat straw on the biomass yield and the production of a cellulolytically active extract employing Pseudomonas aeruginosa. Acta univ [online]. 2017, vol.27, n.5, pp.26-33. ISSN 2007-9621.  https://doi.org/10.15174/au.2017.1276.

Agroindustral residues represents an alternative source for obtaining fermentable products, such as glucose, with higher energy value. Therefore, in this work the isolation of a microorganism with cellulolytic capacity is carried out. A fermentation test of wheat straw with physical pretreatments (grinding), and the combination of a chemical pretreatment - using a saturated solution of Ca(OH)2- with a physical one, combining three particle sizes for the wheat straw were proposed. The results showed that the combination of particle size (141 μm ~ 250 μm) with the chemical pretreatment had a positive effect on biological consumption-degradation of 79.5% and a biomass/substrate yield of 0.49 g/g was observed. Both particle size and alkaline treatment had no significant effect on the cellulolytic activity of the obtained extract (p > 0.05).

Keywords : Cellulases; lime; microfiltration; milling; observed yield.

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