Abstract

GUZMAN-MARTINEZ, María del Carmen; RAMIREZ-ROMERO, Patricia; DAVALOS DE LA CRUZ, Karla  and  AGUILAR SANTAMARIA, María de los Ángeles. Protocol to obtain suspension cells from Artemia franciscana for alkaline unicellular electrophoresis. Hidrobiológica [online]. 2013, vol.23, n.1, pp.133-137. ISSN 0188-8897.

Unicellular electrophoresis is a highly sensitive assay to detect DNA damage caused by various physical, chemical and biological agents. Despite Artemia franciscana is an important experimental model in different areas, there are no studies that refer to genotoxicity in Artemia through this assay. The aim of this study was to establish the protocol to obtain a cell suspension of A. franciscana appropriate in quality and quantity to assess single DNA strand breaks through the comet assay in the future studies.

Keywords : Artemia; cell suspension; comet assay; biomarkers.