Abstract

GUEVARA-VAZQUEZ, E. et al. Identification of yeasts associated with huitlacoche. Rev. Chapingo Ser.Hortic [online]. 2009, vol.15, n.3, pp.225-230. ISSN 2007-4034.

Ustilago maydis (DC) Corda is the phytopathogenic fungus that produces "huitlacoche" on the ears of corn. Its production is of high commercial interest, but yield and quality can be affected by the presence of antagonistic yeasts associated with the gall. Depending on the degree of infection, the galls may have a higher content of yeasts than of U. maydis, which may be a cause of low infestation in the field. The goals of this study were to determine the presence of yeast isolates in a collection of U. maydis and from fresh huitlacoche gall and to explain the association in this structure. To differentiate the two types of microorganisms, the urease test was used and identity was confirmed by molecular techniques and ITS sequencing of the yeast. The results indicated that the yeasts belong to the species Candida railenensis, C. quercitrusa and Pichia guilliermondii and were able to inhibit the growth of U. maydis under laboratory conditions.

Keywords : Ustilago maydis; PCR; antagonic yeast; Candida railenensis; Candida quercitrusa; Pichia guilliermondii.

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