Abstract

SANDOVAL-CANCINO, Gabriela et al. Effect of biofertilizers on tolerance of banana to disinfection and induction of organogenesis. Rev. Chapingo Ser.Hortic [online]. 2013, vol.19, n.2, pp.183-196. ISSN 2007-4034.  https://doi.org/10.5154/r.rchsh.2011.08.042.

The objective of the present study was to evaluate the effect of biofertilizers and culture media on the tolerance to aseptic establishment and induction of organogenesis in banana tree. Explants from suckers of two genotypes of Musa cavendish AAA, cultivars French Clone' and Oaxaqueño Clone', were used. Three applications of biofertilizer were evaluated (1 g of Glomus intraradices, 1 ml of diazotrophic bacterium 11B and the interaction of both microorganisms) and one without biofertilizers. The apices were disinfected and sown in the three culture media: MS without growth regulators, MS added with 22.2 μM of indolacetic acid (IAA) and 22.2 μM of benzylaminopurine (BAP). Measurements were made of the water relations, the content of catalase and peroxidase, the explants with shoots and shoots per explant. In both clones the induction of organogenesis was increased with the addition of BAP and by previous biofertilization of the suckers. In the Oaxaqueño clone, treatments 6, 8 and 11 induced greater organogenesis, with averages between 66 and 71 % of explants with shoots and between 3.16 and 2.28 shoots per explant. For the French clone, treatments 8 and 9 increased the induction of organogenesis with the addition of BAP with averages of 66 to 83 % of explants with shoots and 1.83 to 2.16 shoots per explant. In both genotypes, the biofertilizers propitiated higher percentage of survival, higher tolerance to oxidation and less contamination. In the two genotypes, treatments 1 (Glomus intradices) and 4 (Control) produced less stress in the plants.

Keywords : Musa cavendish; Glomus intraradices; diazotrophs; morphogenesis.

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