Abstract

OJEDA-ZACARIAS, M.C. et al. Rhizogenic response of Pinus maximartinezii Rzedowski. Polibotánica [online]. 2015, n.40, pp.127-135. ISSN 1405-2768.

Pinus maximartinezii Rzedowski is an endemic specie in danger of extinction due to population alteration in the wild and has survived extreme genetic restriction, it is also considered the rarest of pines. In this study rhizogenic response of Pinus maximartinezii sprouts, obtained from zygotic embryos regenerated via organogenesis subjected to in vitro rooting treatments was evaluated. The highest rooting response was obtained with a pulse of 24 h in the presence of 2.0 mg L^-1 of IBA in DCR, GD and MS culture media at 100 and 50% of its original concentration plus 0.5% activated carbon per L^-1. Number of shoots with roots was evaluated after eight weeks, the X² test showed that rooting is independent of culture media, concentration and dependent to the pulse times. Root formation in shoots, allow us to propose this species to be propagated in vitro.

Keywords : buds; rooting; in vitro; Pinus maximartinezii.