Abstract

CISNEROS-MARRERO, Ingrid Viridiana et al. Medium-term in vitro conservation of vanilla (Vanilla planifolia Andrews; Orchidaceae). Polibotánica [online]. 2024, n.57, pp.145-155.  Epub Apr 19, 2024. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.57.8.

Vanilla planifolia Andrews, is a species subject to special protection, which requires the generation of novel conservation strategies, such as medium-term in vitro conservation, allowing for longer intervals between subcultures, while maintaining the ability to reactivate growth rates. In this sense, works dedicated to the medium-term in vitro conservation of vanilla are mainly focused on the use of osmotic agents, nevertheless, growth inhibitors are a promising mechanism whose use should be evaluated, examples of which are abscisic acid (ABA) and chlormequat chloride (CCC), which have been successfully tested in other species, however, so far there are no reports on the effects of their interaction in vanilla. Therefore, the present study aimed to determine the effect of the interaction of different concentrations of ABA (0, 1.5 and 3 mg L-1) and CCC (0, 1, 2, 2, 4 and 6 mg L-1) added to Murashige and Skoog (MS) medium at 75%, on the inhibition of morphogenesis in vitro culture of Vanilla

planifolia microstakes, 1.5 cm in length, subjected to a photoperiod of 16/8 hours light/dark, at 25 ± 2 ºC at 2000 lux light intensity for 200 days, evaluating the total length of the plantlets, the number of shoots, leaves, roots and survival percentage, the best results were obtained in treatment 3.0: 2.0 mg L-1 of ABA/CCC (T 12) because it showed 100% survival, 1.90 cm total length, 0.44 shoots, 1.88 leaves and 1.64 roots. Therefore, the combination of the regulators had a synergistic effect that allowed the medium-term in vitro conservation of V. planifolia by reducing the growth and development of the seedlings without affecting their survival.

Keywords : abscisic acid; chlormequat chloride; reduced growth.