Abstract

SANTIAGO-JERONIMO, Tomasita; CHAVEZ-AVILA, Víctor Manuel; CARBALLAR-HERNANDEZ, Santos  and  GONZALEZ-CUBAS, Rigoberto. Organics compounds in the in vitro propagation of the Mexican endemic orchid Epidendrum falcatum Lindl. Polibotánica [online]. 2024, n.57, pp.157-170.  Epub Apr 19, 2024. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.57.9.

Epidendrum falcatum Lindl. is an endemic orchid of Mexico, with a discontinuous distribution in wild populations growing on limestone rocks. Anthropogenic activities and the demand of collectors have caused a decrease in the wild populations of this species. The objective of this work was to know the effect of two organic compounds on the in vitro propagation of E. falcatum. Seeds from an indehiscent capsule were grown on Murashige and Skoog (MS) medium modified to 50% of macronutrients and added with 100, 200 and 300 mL L-1 and g L-1 of coconut water or banana pulp. The organic compounds promoted germination of E. falcatum; the highest germination percentage was obtained with 100% coconut water. The control presented a greater number of leaves. However, treatments containing plantain significantly (p ≤ 0.01) stimulated the differentiation of the root system and aerial structures. This study represents a successful biotechnological method of in vitro propagation to obtain specimens of E. falcatum and thus counteract the exploitation of wild populations.

Keywords : orchid; growth; conservation; micropropagation.

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