Abstract

HERNANDEZ-MENESES, Eleodoro; RANGEL-ESTRADA, Sandra Eloísa; CANUL-KU, Jaime  and  BARRIOS-GOMEZ, Edwin Javier. In vitro regeneration of Tillandsia takizawae Ehlers & H. Luther. Polibotánica [online]. 2024, n.57, pp.171-181.  Epub Apr 19, 2024. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.57.10.

Tillandsia takizawae Ehlers & H. Luther is an epiphytic bromeliad microendemic from Mexico with showy inflorescences with pink bracts that contrast with the green flowers. These attributes give it potential as an ornamental plant. The conservation of the species and commercial use requires efficient propagation systems that avoid the extraction of plants from their natural habitat. The objectives of this contribution were to establish the optimal conditions for in vitro seed germination and plant regeneration by organogenesis. Seeds were stablished in Murashige and Skoog medium (MS) with half the concentration of salts added with benciladenine (BA) and naftalen acetic acid (NAA). In the elongation and rooting of shoots, the gibberellic acid (GA3) and the indol butiric acid (IBA) were evaluated, respectively. In all treatments an average germination of 97% was obtained. The combination of BA and NAA promoted the induction of shoots in recently germinated seedlings. The highest number

of shoots per explant was 41.4 with 1 mg L-1 of BA and 0.25 mg L-1 of NAA after 12 weeks of culture. Shoots elongation was achieved in the complete MS medium with 1 mg L-1 of GA3 after eight weeks. The best rooting response was with 1 mg L-1 of IBA, which induced the average formation of 7.7 roots in all shoots at four weeks. In the acclimatization of plants the survival was 95 % after four weeks.

Keywords : Bromeliad; endemic; epiphyte; seed germination; organogenesis.

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