Abstract

ARZATE-FERNANDEZ, Amaury M.; HOYOS-BASURTO, Alejandra; VAZQUEZ-GARCIA, Luis M.  and  GUTIERREZ-MARTINEZ, Ma. de Guadalupe. Isozyme characterization of nine botanical varieties of Tigridia pavonia (L. f.) DC. Agrociencia [online]. 2008, vol.42, n.5, pp.519-528. ISSN 2521-9766.

Isozyme banding patterns (IBP) were studied in nine botanical varieties of Tigridia pavonia (L. f.) DC., a native species of México that is highly promising as an ornamental. The varieties were collected in three high-altitude municipalities of the state of México. Tenancingo (2100 m), Temascaltepec (2250 m), and Temoaya ( 2600 m). Leaf tissues of each variety were used as the source for protein extraction. The horizontal starch gel electrophoresis (SGE) technique was used in the runs. With SGE, nine isozymes were tested: aspartate aminotransferase (AAT; EC 2.6.1.1), acid phosphatase (ACP; EC 13.1.3.2), catalase (CAT; EC 1.11.1.6), malate dehydrogenase (MDH; EC 1.1.1.37), 6-phosphogluconate dehydrogenase (PGD; EC 1.1.1.44), phosphoglucose isomerase (PGI; EC 5.3.1.9), phosphoglucomutase (PGM; EC 2.7.5.1), phosphohexose isomerase (PHI; EC 5.3.1.8) and peroxidase (POX; EC 1.11.1.7). Thirty-two bands were observed arranged in 20 IBP: two for AAT, two for ACP, two for CAT, four for MDH, one for PGD, one for POX, three for PGI, three for PHI, and two for PGM. The most polymorphic isozyme was MDH, while PGD and POX did not exhibit polymorphism. The data of seven isozymes were used to calculate genetic relationships with the UPGMA method. T. pavonia varieties could be differentiated from each other, except for the varieties Angeles de Sandra and Carolina de Dulce, which could not be distinguished with the observed IBP. The varieties Gloria and Samaria, collected at 2600 m, had greater genetic variation than those collected at 2100 or 2250 m, possibly attributable to altitude. It is concluded that isozymes can be used to genetically differentiate varieties of T. pavonia.

Keywords : Tigridia pavonia; starch gel electrophoresis; varietal characterization; isozymes; biochemical markers.

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