Abstract

CASTILLO-PEREZ, Luis J.; MALDONADO-MIRANDA, Juan J.; ALONSO-CASTRO, Ángel J.  and  CARRANZA-ALVAREZ, Candy. Effect of 6-benzylaminopurine and potassium nitrate on the in vitro micropropagation of Laelia anceps subsp. anceps (Orchidaceae). Biotecnia [online]. 2020, vol.22, n.1, pp.32-38.  Epub July 03, 2020. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v22i1.1122.

Laelia anceps subsp. anceps is a species of the Orchidaceae family that presents high rates of illegal extraction and trade in several states of the Mexican Republic, and also requires specific nutritional conditions due to its development and distribution characteristics. Therefore, the aim of the present investigation was to establish an in vitro micropropagation protocol comparing the effect of the cytokinin 6-benzylaminopurine (BAP) and potassium nitrate (KNO3). For this, sterile seeds were grown in MS medium without plant growth regulators. After germination, formation of shoots and roots in MS medium was induced in vitroplants supplemented with 0, 2.5, 5.0 and 10 mg L-1 of BAP or 0, 2.5, 5.0 and 10 mg L-1 of KNO3. The results showed that the addition of BAP improved the propagation process in all the treatments analyzed, while the addition of 10 mg L-1 of KNO3 induced the best results by producing 11.4 ± 0.6 roots with the developed of sail, a structure root that favors the water absorption. These results suggest that higher concentration of KNO3 could improve the propagation results in this orchid.

Keywords : conservation; orchids; vegetal biotechnology.