Abstract

SILVA-RODRIGUEZ, David; BELTRAN-GUILARTE, Yayné; ANGULO-MERCADO, Edgardo R.  and  QUILODRAN-TOLOZA, Benita H.. Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium Kinney. Biotecnia [online]. 2021, vol.23, n.2, pp.73-80.  Epub Sep 05, 2022. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v23i2.1405.

Thraustochytrids are characterized by producing omega 3 and 6 type microbial oils. VAL-B1 and EMA-T5 are strains of the same genus that take advantage of pure sources of carbon and residual to produce polyunsaturated fatty acids. Pure (GP) and crude (GC) glycerol were used to compare biomass production and omega 3 and 6 yield and production, using three different substrate concentrations. Using 100 mL of culture at 25 ° C and 180 rpm, the strains were grown for 7 days, then the biomass was centrifuged, and the fatty acid profile quantified by gas chromatography. The results show that EMA-T5 generates a greater amount of biomass at day 5 using 20 g/L of GC with 6.984 ± 0.66 g/L. Regarding the yield and production of omega 3 and 6, VAL-B1 produced the best results with 91.80 ± 8.78 mg/g and 1167.71 ± 70.88 mg/L, being omega 3 the most abundant, especially DHA which presented the highest abundance with 52.72 ± 4.94 % total fatty acids (TFA). It is concluded that EMA-T5 generates higher biomass production using GC and VAL-B1, presents the best performance and omega production results using GP, which indicates that both strains make good use of both the pure and raw sources of glycerol.

Keywords : Polyunsaturated fatty acids; crude glycerol; microbial oils; docosahexaenoic acid.