Abstract

ADAME-GARCIA, Jacel et al. Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper. Biotecnia [online]. 2021, vol.23, n.3, pp.151-157.  Epub Mar 07, 2022. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v23i3.1480.

The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growth-promoting bacteria. This research aimed to isolate growth-promoting bacteria, identify them molecularly, and evaluate their effects on the vegetative development and production of habanero pepper. Five bacterial strains were isolated and identified from samples from different land uses; the agroforestry system recorded on average the most significant amount (3.5x105 CFU g-1) of bacterial strains. Analysis of the 16S ribosomal DNA gene indicated that the bacteria were associated with species belonging to the Bacillus genus; however, the phylogenetic study shows that the identification at a species level is not possible; these strains stimulated better the seedlings growth (6.86, 6.70 and 6.76 cm respectively). In the macro tunnel greenhouse evaluation, the EAV2 strain best stimulated the development of plants (38.63 cm) and produced better fruit weight (6.45 g).

Keywords : Bacillus; biofertilizers; vegetables.

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