Abstract

ANDRADE-ZALDIVAR, H.; KALIXTO-SANCHEZ, M. A.; BARBA DE LA ROSA, A. P.  and  DE LEON-RODRIGUEZ, A.. Expansion of CD34⁺ human hematopoietic cells from umbilical cord blood using roller bottles. Rev. Mex. Ing. Quím [online]. 2014, vol.13, n.2, pp.379-385. ISSN 1665-2738.

Hematopoietic stem cell (HSC) transplantation is limited by the initial CD34⁺ cell content in cord blood units; the aim of this work was the in vitro expansion of HSC to overcome this issue. Supplemented IMDM roller bottle cultures of CD34⁺ cells from human umbilical cord blood were established for hematopoietic progenitor expansion. The maximum total colony forming cells (CFC) expansion was achieved after 5 days of culture, being 16.94±2.82 folds in roller bottles and 17.28±4.47 in static cultures. However, the maximum total cell fold expansion was attained after 10 days of culture. It was of 20.31±6.18 for the roller bottles and 26.45±9.89 for the static cultures. The efficacy of the roller bottles system for short-term cultures of CD34⁺ cells and expanding hematopoietic progenitors in IMDM was demonstrated; encouraging the use of this culture system for other experiments and may be used for future clinical applications.

Keywords : CD34⁺; expansion; hematopoietic stem cell; bone marrow transplantation; cell culture; roller bottles.