Abstract

GALVEZ, R.M.; PABLOS, T.E.; SIGALA, J.C.  and  LARA, A.R.. Co-utilization of glucose and xylose increases growth rate without affecting plasmid DNA yield of engineered E. coli. Rev. Mex. Ing. Quím [online]. 2014, vol.13, n.2, pp.387-391. ISSN 1665-2738.

Escherichia coli strains devoid of PTS system lack of catabolite repression allowing the simultaneous utilization of glucose and other carbohydrates. In this study, the use of xylose and glucose for the production of plasmid DNA (pDNA) was tested using the engineered strain E. coli PTS^- GalP⁺ Δ(recA, deoR). Growth rate and acetate production were similar using glucose or xylose as the carbon source, but pDNA yield (Y_P/x) was lower in cultures with xylose. However, when both carbon sources were co-utilized, growth rate increased by 39 %, and Y_P/x recovered, consequently increasing the specific pDNA production rate by 28 %. Therefore, the used of glucose-xylose mixtures is an attractive alternative for pDNA production.

Keywords : mixed sugars; metabolic engineering; pDNA vaccines; PTS.

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