Abstract

MARTINEZ-TRUJILLO, Ma. A. et al. Optimization of a bacterial biosurfactant production. Rev. Mex. Ing. Quím [online]. 2015, vol.14, n.2, pp.355-362. ISSN 1665-2738.

Bacterial biosurfactant production was optimized by means of Response Surface Methodology (RSM), in which nitrogen and carbon concentrations, as long as the addition of an immiscible substrate, supplemented for increasing the efficiency of biosurfactant biosynthesis, were the evaluated variables. A mixture of yeast extract-NaNO₃, and fructose was used as nitrogen and carbon source, respectively. Under the assayed conditions yeast extract-NaNO₃ relationship and hexadecane concentrations were the factors which had a significant effect on biosurfactant production at flask level. The optimal conditions, estimated by the canonical analysis of the corresponding response surface were used at a 600 mL bioreactor, obtaining a biosurfactant production measured as 74.23 % of emulsification index, which was similar to the estimated by de quadratic model.

Keywords : response surface methodology; canonical analysis; nitrogen and carbon source concentration.