Abstract

VILLARREAL-GARCIA, Laura E. et al. Neolignans from Krameria ramosissima (A. Gray) S. Watson with activity against Porphyromonas gingivalis, cytotoxical and mutagenic evaluations. Rev. mex. cienc. farm [online]. 2014, vol.45, n.2, pp.69-76. ISSN 1870-0195.

Porphyromonas gingivalis, is one of the bacteria associated with periodontal disease that has been related to coronary artery disease, pneumonia, and preeclampsia. The purpose of this study was to evaluate the methanol extract of roots of Krameria ramosissima against P. gingivalis (ATCC 53978), its cytotoxic activity in human fibroblasts (ATCC CRL-7222 274.T Hs) and its mutagenic potential using the Ames test. Asessed concentrations were 500, 400, 300, 200, 150, 100, 75 and 50 µg/mL, with the minimum inhibitory concentration being of 300 µg/mL. By column chromatography were obtained 14 fractions of which the fractions 7 and 9 showed higher inhibitory activity (P <0.05). By means of mass spectrometry we identified two neolignans in the fractions 7 and 9 with molecular weights of 314 and 296 respectively. The extract and the active fractions evaluated at the concentration of 300 µg/mL were negative in the mutagenicity and cytotoxicity tests.

Keywords : Krameria ramosissima; neolignans; Porphyromonas gingivalis; cytotoxic; mutageneses.