Abstract

CORTAZAR FERNANDEZ, Claudia et al. Citotoxicity assessment of different endodontic-use sealing cements in gingival fibroblast cultures. Rev. Odont. Mex [online]. 2013, vol.17, n.1, pp.33-41. ISSN 1870-199X.

The aim of the present study was the in vitro evaluation of the response, within 96 hours, of gingival fibroblast cultures with respect to different endodontic sealers. Results obtained at time intervals of 0, 1, 2, 3, 6, 24, 48, 72 and 96 hours were used to determine sealers' cytotoxicity. Gingival fibroblasts cultures without root canal sealer and with Sealapex were used as negative and positive controls respectively. Results were compared with negative controls and statistically analyzed with t Dunnett test (p ≤ 0.05). Assessed sealing cements were: ProRoot MTA, grey and white, CPM, MTA Angelus, Sealapex and GuttaFlow. Results showed that even though ProRoot MTA (grey and white) MTA Angelus, CPM and GuttaFlow exhibited lower cytotoxic potential than Sealapex, no statistical significant differences were established.

Keywords : Cell culture; endodontic sealers; ProRoot MTA CPM; MTA Angelus.