Abstract

HERNANDEZ-MENDOZA, Fanny et al. Regeneration in vitro of shoots Polianthes tuberosa L. through vegetative buds of the inflorescence and from tissue corm. Nova scientia [online]. 2015, vol.7, n.13, pp.32-47. ISSN 2007-0705.

Tuberose (Polianthes tuberosa L.) is a plant endemic to Mexico, commercially grown for cut flower, and is also used in pharmaceutical and perfume industries. Traditionally, producers propagate this plant using corms, being this cause of the present little genetic variability in the crop and possibly for this reason only cultivars white flowers are known. The objective of this research was to develop a practical and economical method for the regeneration of plantlets from vegetative of the inflorescence and corm. Small buds and corm tissue segments were placed on the surface of the culture medium base GC (personal comunication Guillermo Carrillo), containing inorganic salts of Murashige and Skoog (1962) and also having per liter 50 mL of coconut water, 20 g of sucrose, 6.4 g of agar, being the pH adjusted to 5.7. With this basic medium were prepared a series of culture media containing benzylaminopurine (BAP), naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), indole acetic acid (IAA), and kinetin. The shoot formation that gave rise to small plantlets occurred through the direct regeneration of the flower bud which to grow a plantlets arising only from base of the bud From this region were obtained up to six buds in medium containing BAP, 4.5 mg and NAA, 0.1 mg, being the regeneration of buds higher (56.1%). Corm if the contamination problem was in some cases up to 100% of the cultures. It is important to highlight the importance of achieving massive regeneration in vitro tuberose plant from flower bud.

Keywords : Regeneration efficiency; in vitro; contamination; bud.

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