Abstract

ESPINOSA-HUERTA, Elsa et al. Resistance to Colletotrichum lindemuthianum in transgenic common bean expressing an Arabidopsis thaliana defensin gene. Rev. Mex. Cienc. Agríc [online]. 2013, vol.4, n.7, pp.1027-1042. ISSN 2007-0934.

The defensin gene from Arabidopsis thaliana is an antimicrobial peptide that provides protection to a wide array of pathogens. Transgenic common bean (Phaseolus vulgaris L.) plants of cv. Flor de Mayo Anita constitutively expressing the defensin pdf 1.2 gene were generated by Agrobacterium-mediated transformation via hypocotyls direct organogenic regeneration. The pdf 1. 2 gene under the control of promoter CaMV-35 S was expressed in transformed common bean plants challenged with Colletotrichum lindemuthianum. The observed response was a significant reduction in the formation of lesions and spore mass proliferation in T0, T1 and T3 lines as compared to non-transformed plants. Twenty-seven T3 common bean transgenic plants from 5 independent lines expressing pdf 1.2 gene showed resistance to C. lindemuthianum races 448 and 1472 compared to non-transformed plants. Analysis ofthe level of pdf 1.2 gene expression by Q-PCR indicated that all plants considered resistant or tolerant had similar levels of transcriptional expression as opposed to susceptible plants, which did not have detectable levels of gene expression.

Keywords : Phaseolus vulgaris L.; Agrobacterium tumefaciens; antimicrobial peptide; transformation, pdf 1.2 gene; regeneration.

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