Abstract

ANGELES-NUNEZ, Juan Gabriel; HERRERA-CORREDOR, Carlos  and  MARTINEZ-MARTINEZ, Talina Olivia. Importance of isoform-1 of sucrose synthase in carbon metabolism during seed development Arabidopsis thaliana. Rev. Mex. Cienc. Agríc [online]. 2014, vol.5, n.5, pp.727-739. ISSN 2007-0934.

Sucrose synthase (SUS) is a key enzyme in the metabolism of sucrose. This enzyme catalyzes the reversible conversion of sucrose and UDP to UDP-glucose and fructose. In Arabidopsis, the family SUS of genes consists of 6 members. One of these, the SUS1 gene (At5g20830) is expressed during the development phase of the morphogenesis of the seed, which corresponds to the phase of the massive accumulation of starch and hexoses to date unknown metabolic function has this isoform-1 in the synthesis of these carbon compounds. In order to provide elements that contribute to determine the roles of this enzyme (SUS1) in seed development was carried out a functional analysis, which comprises the biochemical characterization of a null allele of this isoform. The results show the importance of this enzyme (SUS1) for sucrose metabolism in Arabidopsis seeds in the development stage. Moreover, the mutation affects mainly SUS1 hexoses/sucrose ratio; which results in a significant accumulation of fatty acids and of transitory starch in the early stages of seed development decreased.

Keywords : starch; fatty acid; isoform-1 sucrose synthase; developing seeds.