Abstract

OCHOA FONSECA, Lyda Esperanza et al. Effect of mineral preparations on growth and in vitro development of Moniliophthora roreri (Cif. & Par.) Evans. Rev. Mex. Cienc. Agríc [online]. 2015, vol.6, n.5, pp.1065-1075. ISSN 2007-0934.

The present study was developed with the aim to evaluate the fungicidal activity of mineral preparations on Moniliophthora roreri under laboratory conditions: broth visosa (CV), Bordeaux mixture plus potassium permanganate (CBP), Bordeaux mixture plues zinc sulfate and sulfate magnesium (CBS), broth sodium bicarbonate (BiS), broth silicosulfocálcico (SSC) and calcium polysulfide (PC). Two bioassays were performed; in the first, the inhibitory effect on fungal growth was assessed by the agar diffusion method, the compounds were tested at five concentrations (50, 40, 30, 20 and 10% v / v). In the second, through the cultivation technique cultivation in liquid medium in test tubes, the effect on formation and germination of fungus spores was determined; The culture medium consisted of a solution of cocoa extract plus water (1:1 v / v) to which fungus spores and 50% (v / v) ofeach mineral preparation were added. With the exception of BiS at 10%, mycelial growth was inhibited completely or partially by the other mineral preparations, showing statistically significant differences with control. SSC and PC preparations were the most effective in inhibiting 100% mycelial growth even at 10% concentration. The test showed that in liquid medium at 96 hours the SSC and PC preparations were the most effective, reducing up to 98.2 and 79.9% of spores respectively; spore germination was only present in control treatment. Spore formation in BiS treatments at all concentrations and in control was observed, but no mineral preparations allowed spore germination.

Keywords : Theobroma cacao L.; Moniliasis; organic agriculture.

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