Abstract

IBARRA-LOPEZ, Alejandro; OJEDA-ZACARIAS, Ma. del Carmen; GARCIA-ZAMBRANO, Eduardo Alejandro  and  GUTIERREZ-DIEZ, Adriana. In vitro induction seedlings to two cultivars Mexican race avocado Persea americana var. drymifolia Schltdl. & Cham. Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.2, pp.337-347. ISSN 2007-0934.

One approach micropropagation avocado rootstock is the multiplication of commercial interest. In the present study aimed to identify the suitable culture medium for the stages of establishment and induction of buds of cultivars Huevo de Toro and butter, for which settled internodal segments with axillary buds (micropiles) in the media MS, DCR and Yasuda culture, added with 2 mg L-1 of 6-benzylaminopurine (BAP), 0.3 mg L-1 indolbutirico acid (AIB), 2 g L-1 of oxytetracycline and 2 g L-1 benomyl. The variables contamination, oxidation and viability of the explants were evaluated 14 days after sowing. The culture medium was Yasuda stage suitable for aseptic accommodation which induced the most viable explants (> 40%). These were subcultured in mass seedlings induction, MS, DCR and Yasuda supplemented with 20% coconut water (AC), 2 mg L-1 BAP, 0.5 mg L-1 of gibberellic acid (AG3) and 0.01 mg L-1 indoleacetic acid (AIA) (M1, M2 and M3); 2 g L-1 casein hydrolyzate (CH), 1 mg L-1 BAP and 0.3 mg L-1 AIB (M4, M5 and M6). The number of seedlings s per explants, seedlings length and number of leaves at 60 and 90 days subculture was evaluated. In the growing Huevo de Toro, M1 and M2 are satisfactory means for the proliferation of seedlings values 1.19-1.5, 1.07-1.09 cm seedlings length, and the number of sheets 1.21. For the cultivar butter, M3, was the best for the variables: number of seedlings (1.89-214), seedlings length (1.21-1.23 cm) and number sheets (1.21-2.38).

Keywords : Persea americana Mill.; in vitro; coconut water; hydrolyzed casein; organogenesis.