Abstract

AGUILAR-MORALES, M. A.; LAGUNA-CERDA, A.; VENCES-CONTRERAS, C.  and  LEE-ESPINOSA, H. E.. Analysis seeds Encyclia adenocaula (La Llave & Lex.) Schltr (Orchidaceae) for ex situ conservation. Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.7, pp.1741-1747. ISSN 2007-0934.

Seeds Trompillo (Encyclia adenocaula (La Llave & Lex.) Schltr. Orchidaceae) from mature capsules of a population, were analyzed for: size, structure, weight, number of seeds per gram and viability by tetrazolium test with without pretreatment of Ca(OCl)2 and Tween, asymbiotic test germination conditions in vitro to compare the viability of them. Germination was evaluated in germination percentage and days in three semi-solid culture media (MS + CV; MS + banana extract; MS + coconut water). The seed size was 0.56±0.08 mm long by 0.09±0.01mm wide, covering an area of 3.25±0.5 mm2, with an approximate weight of 1.6±0.08μg, registering 63 500 seeds per gram. The percentage of viability by tetrazolium test showed statistically significant differences (p≤0.05) between treatments, excelling treatments Tetrazolium 1 % with and without Tween with 50.3 and 48.2% respectively, pretreatment with Ca(OCl)2 affected directly the viability of the seeds. In asymbiotic germination were significant statistical differences (p≤ 0.05) between treatments, highlighting MS + CV with 56.9% germination at 30 days, followed by medium MS + coconut water with 31.4% germination to 55.7 days, finally medium MS + banana extract with 23% to 59.1 days.

Keywords : coconut water; banana extract; germination; mature capsules; trompillo.