Abstract

DIAZ LEZCANO, Maura Isabel et al. Phosphatase-alkaline activity and growth of rice with biological inoculation and micronutrients. Rev. Mex. Cienc. Agríc [online]. 2020, vol.11, n.3, pp.481-492.  Epub Aug 30, 2021. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v11i3.1873.

Inoculation with growth promoting bacteria in crops is an alternative to reduce the use of nitrogen fertilizers and production costs. The objective of this research was to evaluate the effect of inoculating Oryza sativa L. rice seeds with the bacteria Azospirillum brasilense on the enzymatic activity of alkaline phosphatase and its growth. The research was carried out at the Faculty of Agrarian Sciences and the Faculty of Chemical Sciences of the National University of Asuncion, Paraguay. A completely randomized design with five treatments and five repetitions was used. Treatments consisted of T1 (Absolute Control): untreated rice seeds, T2 (Co+Mo): rice seeds treated with cobalt plus molybdenum, T3 (Az): rice seeds inoculated with A. brasilense, T4 (Az+1F): rice seeds inoculated with A. brasilense plus one foliar application of the inoculant and T5 (Az+2F): rice seeds inoculated plus two foliar applications. Anava and Tukey’s test (p<0.05) were applied for the analysis of agronomic variables and the Kruskal-Wallis test (p<0.05) for enzymatic evaluations. The variables evaluated were root length (cm), dry mass of aerial parts and roots (g), and alkaline phosphatase enzyme activity (μg de PNP g-1 soil h-1). No significant differences were observed between treatments with Azospirillum brasilense regarding dry mass. Phosphatase activity in treatments with A. brasilense ranged from 1 655.49 in T3 to 7 429.55 μg of PNP g-1 soil h-1 in T4. The use of cobalt and molybdenum-based micronutrients led to greater root growth.

Keywords : Azospirillum brasilense; Oryza sativa L.; enzymatic activity; inoculation.

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