Abstract

VAZQUEZ-GARCIDUENAS, Ma. Soledad; MELENDEZ-CEJA, Silvia Cristina  and  VAZQUEZ-MARRUFO, Gerardo. A multiplex PCR assay for detection of genus Salmonella, subspecies I, and serotype Typhimurium in milk. Rev. mex. de cienc. pecuarias [online]. 2015, vol.6, n.3, pp.329-335. ISSN 2448-6698.

Gastrointestinal diseases caused by foodstuffs contaminated with Salmonella enterica implicate important social and economic issues, making it necessary to have fast, sensitive, and specific systems for opportune detection of the pathogen. An endpoint PCR protocol for identification of S. enterica in milk is presented using primer pairs previously validated as specific to the genus Salmonella (STM3098-f2/STM3098-r2), subspecies enterica (subspecies I) (STM4057-f/STM4057-r), and serotype Typhimurium (STM4497-f/ STM4497-r). This protocol allowed for detection of 1 CFU/25 mL of spiked milk after a 12 h pre-enrichment period by means of simple and multiplex PCR assay with the three used primer pairs. The designed protocol can be applied in sanitary survey programs for detection of S. enterica in milk.

Keywords : Salmonella enterica; Milk; Multiplex PCR.