Abstract

PACHECO-TORRES, Irene et al. Exploring bovine fecal bacterial microbiota in the Mapimi Biosphere Reserve, Northern Mexico. Rev. mex. de cienc. pecuarias [online]. 2022, vol.13, n.4, pp.910-927.  Epub Nov 11, 2022. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v13i4.6138.

In Mexico, information on the bovine fecal microbiota (Bos taurus) is scarce. The present study describes the diversity and abundance of bacteria in fecal samples from rangeland bovines, collected in the Mapimi Biosphere Reserve in the central part of the Chihuahuan desert. Fecal samples were analysed using high-throughput next generation massive sequencing using V3-V4 16S rRNA on Illumina Miseq. A total of 17 phyla, 24 classes, 33 orders, 50 families, 281 genera, and 297 species were identified. Firmicutes and Verrucomicrobia were the most abundant phyla. The most abundant genera were Sporobacter, PAC000748_g (genera into the Ruminococcaceae family) and Eubacterium_g23. Three genera (Clostridium, Corynebacterium and Fusobacterium) and one species (Campylobacter fetus) potentially pathogenic bovine bacteria were registered. This information represents a bacteriological baseline for monitoring the grazing bovine intestinal health status, and to trace possible interactions with the fecal microbiota of native roaming wildlife in the area.

Keywords : Bos taurus; Campylobacter fetus; Bacterial diversity; 16S rRNA gene; Massive sequencing.