Abstract

SALINAS ESTRELLA, Elizabeth et al. Antigen production and standardization of an in-house indirect ELISA for detection of antibodies against Anaplasma marginale. Rev. mex. de cienc. pecuarias [online]. 2022, vol.13, n.4, pp.1079-1094.  Epub Nov 11, 2022. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v13i4.5976.

Serologic tests are important for the detection of specific antibodies against infectious agents. Commercial indirect ELISA are costly and usually as effective as in-house ELISAs. In the present work, it was prepared a batch of Anaplasma marginale crude antigen from infected blood, and tested it against official positive and negative serum controls and compared with an old batch of antigen. The new antigen batch showed an efficiency similar to the old batch. The sensitivity of the test was comparable between the new and old batches. Both, new and old antigen lots are being used at an excess. The new antigen lot is large enough to run thousands of tests at a more affordable price than commercial kits.

Keywords : Bovine anaplasmosis; Indirect ELISA; Serologic diagnostics.