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Revista mexicana de ciencias pecuarias
On-line version ISSN 2448-6698Print version ISSN 2007-1124
Abstract
NAVARRO-LOPEZ, Roberto et al. Genetic characterization of bovine viral diarrhea virus 1b isolated from mucosal disease. Rev. mex. de cienc. pecuarias [online]. 2023, vol.14, n.1, pp.248-259. Epub Mar 24, 2023. ISSN 2448-6698. https://doi.org/10.22319/rmcp.v14i1.6200.
This report describes a fatal case of mucosal disease in a two-year-old bull. For causal agent detection, scab, whole blood, and feces samples were tested by RT-PCR, PCR, ELISA, and viral isolation. RT-PCR positive amplification was obtained in blood samples for bovine viral diarrhea virus (BVDV). Viral isolation from the scab samples confirmed BVDV as the causative agent of the clinical manifestations. Subsequently, genetic characterization based on phylogenetic analysis of three partial sequences revealed the presence of BVDV subgenotype 1b in analyzed samples. Due to the development of clinical manifestation named mucosal disease, these findings suggest the detection of BVDV persistently infected (PI) bull; therefore, these results demonstrate the importance of establishing BVDV control programs that rely on testing the presence of PI in cattle from Mexico.
Keywords : Bovine viral diarrhea virus; Cattle; Mucosal disease; Persistent infection; Mexico.