Abstract

VALLE-DE LA PAZ, M. et al. Species of Lasiodiplodia in lima ‘Persa’ (Citrus latifolia Tanaka) in Morelos, México. Revista bio ciencias [online]. 2019, vol.6, e595.  Epub Oct 02, 2020. ISSN 2007-3380.  https://doi.org/10.15741/revbio.06.e595.

Samples of ‘Persian’ lime trees, from 5 to 8 years old, with symptoms of gummosis, cankers and dieback of branches were collected in orchards in the municipalities of Tlaltizapan, Ayala, Coatlan del Rio and Amayuca in the state of Morelos, Mexico, during August 2015. The samples were processed by routine procedures and sown in potato dextrose-agar and purified by means of hypha tips. The isolated pathogens were inoculated in healthy plants in order to evaluate their pathogenicity. Morphological and molecular identification was carried out and a Bayesian phylogenetic tree was constructed with clades grouping. Based on morphological characters, Lasiodiplodia sp. was identified. The molecular analysis allows to determine 16 isolates, which were grouped into 4 clades and three species, Lasiodiplodia citricola, L. pseudotheobromae and L. theobromae, which are considered as new reports, causing disease in ‘Persian’ lime for the State of Morelos, Mexico.

Keywords : Molecular identification; Lasiodiplodia theobromae; Lasiodiplodia pseudotheobromae; Lasiodiplodia citricola.