Abstract

CARRILLO-BENITEZ, María Guadalupe; RODRIGUEZ-DE LA O, José Luis  and  ALVAREZ-MOCTEZUMA, José Guadalupe. In vitro morphogenesis in Pseudotsuga menziesii var. glauca. Rev. Chapingo ser. cienc. for. ambient [online]. 2011, vol.17, n.2, pp.273-282. ISSN 2007-4018.  https://doi.org/10.5154/r.rchscfa.2010.04.024.

This study assessed the morphogenic response of in vitro cultured zygotic embryos obtained from Pseu-dotsuga menziesii var. glauca seed collected in Tlaxcala, Mexico and stored for one year. Seeds were disinfected with detergent and H₂O₂ (3 % v/v) for 48 h under stirring at 50 rpm and cultured on Murashige and Skoog (1962) medium without regulators. Germination occurred after seven days and then the explants were subcultured on MS medium with 2,4-D (3 mg·L^-1) and BA (1 mg·L^-1). With the calluses obtained on HS medium, three ABA concentrations intended to promote formation of embryonic structures were evaluated. The best treatment was with a concentration of 10.0 mgL^-1 (P<.0001). The best plantlet development curred using Murashige and Skoog (1962) medium with 6% sucrose. Mycorrhizae were used to improve plantlet adaptation to soil. No roots formed.

Keywords : Embryo; callus; plantlets; growth regulators.