Abstract

MURRIETA-HERNANDEZ, Dulce Ma. et al. Effect of culture medium on development of Suillus granulatus (L.) Roussel and S. brevipes (Pk.) Kuntze. Rev. Chapingo ser. cienc. for. ambient [online]. 2014, vol.20, n.1, pp.101-107. ISSN 2007-4018.  https://doi.org/10.5154/r.rchscfa.2013.06.021.

The mycelial growth rates of ectomycorrhizal fungi Suillus granulatus and S. brevipes were assessed in three culture media (PDA, BAF and MMN) at two pH values (4.8 and 5.8) in order to select the best culture medium. The strains were isolated from sporocarps collected in a Pinus hartwegii forest in Cofre de Perote National Park, Veracruz, Mexico. Significant differences (Tukey, P ≤ 0.05) in the growth area of both species were found. The highest values were recorded in the PDA medium. Concerning the pH values tested, there were no significant differences. Each of the tested media can be used for culturing the S. granulatus and S. brevipes strains, depending on the objectives. The PDA medium was the best substrate for the growth of the strains. We suggest using the BAF medium for mass production of mycelium for inoculum, and the MMN medium for either strain maintenance or mycorrhization testing.

Keywords : Boletes; temperate forest; ectomycorrhizae; edible mushrooms; pines.