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Botanical Sciences
On-line version ISSN 2007-4476Print version ISSN 2007-4298
Abstract
ŠUTKOVIć, Jasmin; KARIć, Ajdina and YILDIRIM, Ahmet. In silico identification and expression analysis of Metal-nicotianamine transporter (YSL3) and Oligopeptide transporter 3 (OPT3) under Cd stress in Brassica oleracea var. acephala. Bot. sci [online]. 2020, vol.98, n.4, pp.516-523. Epub Feb 09, 2021. ISSN 2007-4476. https://doi.org/10.17129/botsci.2628.
Background:
Metal-nicotianamine transporter (YSL) family protein belongs to the oligopeptide heavy metal transporter group, as characterized in Arabidopsis thaliana. Oligopeptide transporters (OPTs) are a group of membrane-localized proteins, involved in different transport mechanisms, contributing to nitrogen mobilization, glutathione transport and long-distance metal distribution. Metal-nicotianamine transporter gene 3 (YSL3) incorporates the oligopeptide transporter domain, found to transfer several heavy metals in diverse plant species, and among them cadmium transport in Brassica oleracea.
Objective:
To evaluate and confirm the expression of Metal-nicotianamine transporter (YSL3) under cadmium stress.
Studied species:
Brassica oleracea var. acephala
Study site and dates:
Brassica oleracea var. acephala samples were collected from Blagaj region, Bosnia and Herzegovina.
Methods:
Through a simple bioinformatic approach the interactome partner of Metal-nicotianamine transporter (YSL3) was discovered and annotated. Oligopeptide transporter 3 (OPT3) and Metal-nicotianamine transporter (YSL3) genes were checked for expression levels under cadmium stress.
Results:
We have identified a strong interacting partner of YSL3, later confirmed as Oligopeptide transporter 3 (OPT3) protein in Brassica oleracea. The in vitro expression analysis by using a qRT-PCR revealed a significant upregulation of YSL3 and OPT3, during Cd stress.
Conclusions:
These findings indicate that the represented in-silico approach, followed by in vitro gene expression study, successfully confirmed YSL3 and identified OPT3 as a new gene, in correlation to cadmium stress.
Keywords : Bioinformatics; Gene expression; Heavy metals; Uncharacterized protein.