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Ecosistemas y recursos agropecuarios
On-line version ISSN 2007-901XPrint version ISSN 2007-9028
Abstract
ESPINO-CASTILLO, Daniel Alejandro et al. Evidence of a 20 kDa vegetative storage protein in pecan (Carya illinoensis Koch) root during the dormancy stage. Ecosistemas y recur. agropecuarios [online]. 2018, vol.5, n.14, pp.309-317. ISSN 2007-901X. https://doi.org/10.19136/era.a5nl4.1616.
An analysis of total soluble proteins and vegetative storage proteins (VSPs) in the tissues of pecan (Carya illinonensis Koch var. Western) trees in dormancy and production was carried out. Total soluble proteins were determined using the Bradford method, by means of electrophoresis in 15% polyacrylamide gel, to detect the VSPs present in the plant tissues. The results indicate that the branches and shoots of annual growth were the organs with the highest concentration of total soluble proteins in dormancy, while in production there was no significant difference in concentration between the different tissues studied. A 20-kDa polypeptide with characteristics of a VSP was detected in the root in the dormancy stage.
Keywords : Perennial organs; fruit trees; phenology; proteins; VSP.