Abstract

BIGURRA-QUINTERO, Uldarico; MENDOZA-VILLARREAL, Rosalinda; ROBLEDO-TORRES, Valentín  and  PAREDES-JACOME, José Rafael. Kinetics of in vitro growth of Brevibacillus brevis in different culture media. Ecosistemas y recur. agropecuarios [online]. 2020, vol.7, n.2, 00009.  Epub Dec 22, 2020. ISSN 2007-901X.  https://doi.org/10.19136/era.a7n2.2310.

The development of techniques for the reproduction of soil beneficial rhizobacteria opens up a range of alternatives for your research. The ignorance of chemical and physical conditions of an optimal means for development Brevibacillus brevis generates this research by evaluating five liquid culture media (SM 1%, SM 0.5%, SM 0.1%, LB and NFb), three pHs (6, 7 and 8) and two incubation times (48 and 96 h). The objective of this work was to evaluate an optimal medium for the reproduction of the bacteria at the laboratory level. The methodology included processing, standardizing of culture media, reactivation of the strain, Gram staining, inoculation, incubation for reproduction and bacterial count. The triple interaction showed that the medium SM 1% (Sábila and Melaza), with pH 7 and 48 h had the best results, compared to the other media and conditions with 15.38 x 1011 CFU mL-1.

Keywords : Aloe; Bacillus; molasses; rhizobacteria; substrates.